Detailed information of TA system TA212336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2832159..2832730
• Replicon: chromosome
• Accession: NZ_CP080759
• Organism: Enterococcus faecalis strain M817

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K2228_RS13905
• Protein ID: WP_002354774.1
• Coordinates: 2832159..2832500 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: K2228_RS13910
• Protein ID: WP_002354773.1
• Coordinates: 2832500..2832730 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2228_RS13900 (2828174)	2828174..2831788	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
K2228_RS13905 (2832159)	2832159..2832500	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K2228_RS13910 (2832500)	2832500..2832730	-	231	WP_002354773.1	hypothetical protein	Antitoxin
K2228_RS13915 (2832904)	2832904..2833119	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
K2228_RS13920 (2833258)	2833258..2834250	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
K2228_RS13925 (2834417)	2834417..2835055	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
K2228_RS13930 (2835740)	2835740..2837356	+	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T212336 WP_002354774.1 NZ_CP080759:c2832500-2832159 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T212336 NZ_CP107003:1800235-1800486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT212336 WP_002354773.1 NZ_CP080759:c2832730-2832500 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT212336 NZ_CP107003:1800483-1800737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812