Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1496023..1497160 Replicon chromosome
Accession NZ_CP080759
Organism Enterococcus faecalis strain M817

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag K2228_RS07335 Protein ID WP_002401483.1
Coordinates 1496023..1496886 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag K2228_RS07340 Protein ID WP_000301765.1
Coordinates 1496888..1497160 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K2228_RS07300 (1491374) 1491374..1492762 - 1389 Protein_1408 ABC transporter ATP-binding protein -
K2228_RS07305 (1492819) 1492819..1493499 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
K2228_RS07310 (1493554) 1493554..1493847 + 294 Protein_1410 ABC transporter ATP-binding protein -
K2228_RS07315 (1493840) 1493840..1494148 + 309 Protein_1411 ABC transporter ATP-binding protein -
K2228_RS07320 (1494204) 1494204..1494884 - 681 WP_010710189.1 IS6-like element IS1216 family transposase -
K2228_RS07325 (1494958) 1494958..1495245 - 288 Protein_1413 DnaJ domain-containing protein -
K2228_RS07330 (1495265) 1495265..1495582 - 318 WP_002333463.1 hypothetical protein -
K2228_RS07335 (1496023) 1496023..1496886 - 864 WP_002401483.1 zeta toxin family protein Toxin
K2228_RS07340 (1496888) 1496888..1497160 - 273 WP_000301765.1 antitoxin Antitoxin
K2228_RS07345 (1497177) 1497177..1497392 - 216 WP_002295735.1 peptide-binding protein -
K2228_RS07350 (1497491) 1497491..1498387 - 897 WP_002387620.1 ParA family protein -
K2228_RS07355 (1498490) 1498490..1498750 - 261 Protein_1419 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
K2228_RS07360 (1498920) 1498920..1499657 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
K2228_RS07365 (1499782) 1499782..1499877 - 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
K2228_RS07370 (1499946) 1499946..1500068 - 123 Protein_1422 peptide-binding protein -
K2228_RS07375 (1500188) 1500188..1501714 - 1527 WP_256128437.1 type IA DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / cat - 1492819..1516256 23437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-213)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32486.06 Da        Isoelectric Point: 6.9965

>T212331 WP_002401483.1 NZ_CP080759:c1496886-1496023 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T212331 NZ_CP107003:714669-714965 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT212331 WP_000301765.1 NZ_CP080759:c1497160-1496888 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT212331 NZ_CP107003:714967-715362 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References