Detailed information of TA system TA212327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 429190..429724
• Replicon: chromosome
• Accession: NZ_CP080759
• Organism: Enterococcus faecalis strain M817

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: K2228_RS02225
• Protein ID: WP_002360769.1
• Coordinates: 429449..429724 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: K2228_RS02220
• Protein ID: WP_002369771.1
• Coordinates: 429190..429456 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2228_RS02185 (425240)	425240..425833	+	594	WP_002414802.1	replication-relaxation family protein	-
K2228_RS02190 (425839)	425839..426327	+	489	WP_010710133.1	hypothetical protein	-
K2228_RS02195 (426919)	426919..427185	+	267	WP_250374031.1	hypothetical protein	-
K2228_RS02200 (427218)	427218..427718	+	501	WP_002360775.1	DnaJ domain-containing protein	-
K2228_RS02205 (427731)	427731..427979	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
K2228_RS02210 (428055)	428055..428471	+	417	WP_010710134.1	single-stranded DNA-binding protein	-
K2228_RS02215 (428495)	428495..429079	+	585	WP_002377949.1	thermonuclease family protein	-
K2228_RS02220 (429190)	429190..429456	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
K2228_RS02225 (429449)	429449..429724	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
K2228_RS02230 (429915)	429915..430559	-	645	WP_002377952.1	IS6 family transposase	-
K2228_RS02235 (430692)	430692..431574	+	883	Protein_416	IS256 family transposase	-
K2228_RS02240 (431879)	431879..432916	-	1038	WP_002355369.1	PTS sugar transporter subunit IIC	-
K2228_RS02245 (432942)	432942..433880	-	939	WP_002370935.1	2-dehydropantoate 2-reductase	-
K2228_RS02250 (434008)	434008..434337	-	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
K2228_RS02255 (434367)	434367..434552	+	186	WP_002358660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	bsh / cylR2 / cylR1 / cylL / cylS / cylM	421847..441238	19391

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T212327 WP_002360769.1 NZ_CP080759:429449-429724 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T212327 NZ_CP107002:c4215162-4214989 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT212327 WP_002369771.1 NZ_CP080759:429190-429456 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT212327 NZ_CP107002:c4215486-4215190 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7