Detailed information of TA system TA212322

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 1185955..1186624
• Replicon: chromosome
• Accession: NZ_CP080635
• Organism: Shewanella putrefaciens strain YZ-J

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: K3G22_RS19485
• Protein ID: WP_014610119.1
• Coordinates: 1186184..1186624 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: -
• Locus tag: K3G22_RS05365
• Protein ID: WP_014610118.1
• Coordinates: 1185955..1186203 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3G22_RS05340 (K3G22_05340)	1181578..1182381	+	804	WP_198779862.1	prolipoprotein diacylglyceryl transferase	-
K3G22_RS05345 (K3G22_05345)	1182384..1183178	+	795	WP_011790287.1	thymidylate synthase	-
K3G22_RS05350 (K3G22_05350)	1183295..1184464	+	1170	WP_011790286.1	Na+/H+ antiporter NhaA	-
K3G22_RS05355 (K3G22_05355)	1184467..1184853	+	387	WP_011790285.1	hypothetical protein	-
K3G22_RS05360 (K3G22_05360)	1184961..1185869	+	909	WP_011790284.1	transcriptional activator NhaR	-
K3G22_RS05365 (K3G22_05365)	1185955..1186203	+	249	WP_014610118.1	succinate dehydrogenase assembly factor 2	Antitoxin
K3G22_RS19485	1186184..1186624	+	441	WP_014610119.1	hypothetical protein	Toxin
K3G22_RS05370 (K3G22_05370)	1186661..1188274	-	1614	WP_011790281.1	L-aspartate oxidase	-
K3G22_RS05375 (K3G22_05375)	1188464..1189042	+	579	WP_011790280.1	RNA polymerase sigma factor RpoE	-
K3G22_RS05380 (K3G22_05380)	1189069..1189689	+	621	WP_011790279.1	anti sigma-E factor RseA C-terminal domain-containing protein	-
K3G22_RS05385 (K3G22_05385)	1189702..1190634	+	933	WP_011790278.1	MucB/RseB C-terminal domain-containing protein	-
K3G22_RS05390 (K3G22_05390)	1190645..1191118	+	474	WP_025007378.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (6-135)

Antitoxin

• Flavinator of succinate dehydrogenase (7-78)

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 17313.52 Da
• Isoelectric Point: 9.2750

>T212322 WP_014610119.1 NZ_CP080635:1186184-1186624 [Shewanella putrefaciens]
VEEQHHSFSVKASFDQRLSLVVFMCVCSSSFLIWPYTEHWLVALLRLGLFLLSVSFLIWQLWRLKDWRLSFMLNGKGDGR
LSTGEHFRILRRTWVTPFVCMIYIDVEEKTRLVLLWADMFTGVDYRHLCRLLLKAKMLQAKSQAEI

Nucleotide

• Length: 441 bp

>T212322 NZ_CP107002:3743321-3743662 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9545.15 Da
• Isoelectric Point: 4.5994

>AT212322 WP_014610118.1 NZ_CP080635:1185955-1186203 [Shewanella putrefaciens]
MELMNIARVRWACRRGMLELDVLFQPFVENIYQDLSDIDKASFIRLLECEDPELFAWFMGHEACPDAELARMVVKVRGRA
AP

Nucleotide

• Length: 249 bp

>AT212322 NZ_CP107002:3742983-3743300 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA