Detailed information of TA system TA212316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-YefM
• Location: 391539..392089
• Replicon: chromosome
• Accession: NZ_CP080633
• Organism: Shewanella putrefaciens strain YZ08

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: K2227_RS01820
• Protein ID: WP_220591777.1
• Coordinates: 391539..391838 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: K2227_RS01825
• Protein ID: WP_220591778.1
• Coordinates: 391847..392089 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2227_RS01805 (K2227_01805)	387587..388078	-	492	WP_220591775.1	GNAT family N-acetyltransferase	-
K2227_RS01810 (K2227_01810)	388738..389571	+	834	WP_220591776.1	metal-binding protein	-
K2227_RS01815 (K2227_01815)	390090..391103	+	1014	WP_107946627.1	IS110 family transposase	-
K2227_RS01820 (K2227_01820)	391539..391838	-	300	WP_220591777.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K2227_RS01825 (K2227_01825)	391847..392089	-	243	WP_220591778.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K2227_RS01830 (K2227_01830)	392368..393111	-	744	WP_220591779.1	hypothetical protein	-
K2227_RS01835 (K2227_01835)	393140..394396	-	1257	WP_220591780.1	beta-ketoacyl-ACP synthase	-
K2227_RS01840 (K2227_01840)	394397..395122	-	726	WP_220591781.1	3-ketoacyl-ACP reductase FabG2	-
K2227_RS01845 (K2227_01845)	395288..395821	-	534	WP_220591782.1	hotdog family protein	-
K2227_RS01850 (K2227_01850)	395826..397025	-	1200	WP_259399964.1	beta-ketoacyl-[acyl-carrier-protein] synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	390090..391103	1013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-90)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11524.37 Da
• Isoelectric Point: 9.9595

>T212316 WP_220591777.1 NZ_CP080633:c391838-391539 [Shewanella putrefaciens]
MKNKRYKLSKLAQTHLLKIKDYTIQHFSESQWHKYKQSLISGLKMLADNPGLGRSCDDIYPNGFYFPIGKHTAYFTKEDD
FILIVALLGQPQLPQNHLK

Nucleotide

• Length: 300 bp

>T212316 NZ_CP107002:2247036-2247323 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8906.07 Da
• Isoelectric Point: 4.7483

>AT212316 WP_220591778.1 NZ_CP080633:c392089-391847 [Shewanella putrefaciens]
VQTLTANDAKRNFGELLLNAQREPIKISKNSKDAVVVMSIHDFEQLEAMKADYLKHCFNAAKEDLAQGNVVDGEDFLNAL

Nucleotide

• Length: 243 bp

>AT212316 NZ_CP107002:2246797-2247036 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA