212306

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518143..518779 Replicon chromosome
Accession NZ_CP080629
Organism Bacillus subtilis strain YPS-32

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag K3G18_RS02630 Protein ID WP_003156187.1
Coordinates 518429..518779 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag K3G18_RS02625 Protein ID WP_003225183.1
Coordinates 518143..518424 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3G18_RS02605 (514502) 514502..515101 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
K3G18_RS02610 (515196) 515196..515561 + 366 WP_015252768.1 holo-ACP synthase -
K3G18_RS02615 (515727) 515727..516743 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
K3G18_RS02620 (516858) 516858..518027 + 1170 WP_032722929.1 alanine racemase -
K3G18_RS02625 (518143) 518143..518424 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
K3G18_RS02630 (518429) 518429..518779 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K3G18_RS02635 (518894) 518894..519718 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
K3G18_RS02640 (519723) 519723..520088 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
K3G18_RS02645 (520092) 520092..520493 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
K3G18_RS02650 (520505) 520505..521512 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
K3G18_RS02655 (521574) 521574..521903 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
K3G18_RS02660 (521900) 521900..522382 + 483 WP_003234299.1 anti-sigma B factor RsbW -
K3G18_RS02665 (522348) 522348..523136 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
K3G18_RS02670 (523136) 523136..523735 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T212306 WP_003156187.1 NZ_CP080629:518429-518779 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T212306 NZ_CP107002:648758-649072 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT212306 WP_003225183.1 NZ_CP080629:518143-518424 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT212306 NZ_CP107002:649069-649485 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References