Detailed information of TA system TA212301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3262716..3263363
• Replicon: chromosome
• Accession: NZ_CP080626
• Organism: Pseudomonas sp. S11A 273

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3F43_RS14455
• Protein ID: WP_045182275.1
• Coordinates: 3262947..3263363 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K3F43_RS14450
• Protein ID: WP_045182277.1
• Coordinates: 3262716..3262946 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3F43_RS14430 (K3F43_14430)	3258157..3259455	-	1299	WP_259407434.1	precorrin-3B synthase	-
K3F43_RS14435 (K3F43_14435)	3259612..3260823	+	1212	WP_101292302.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
K3F43_RS14440 (K3F43_14440)	3260816..3261913	+	1098	WP_101292300.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
K3F43_RS14445 (K3F43_14445)	3261910..3262620	+	711	WP_045182278.1	cobalt-precorrin-6A reductase	-
K3F43_RS14450 (K3F43_14450)	3262716..3262946	+	231	WP_045182277.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K3F43_RS14455 (K3F43_14455)	3262947..3263363	+	417	WP_045182275.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K3F43_RS14460 (K3F43_14460)	3263584..3264021	+	438	WP_045182273.1	NfeD family protein	-
K3F43_RS14465 (K3F43_14465)	3264045..3264902	+	858	WP_045182271.1	SPFH domain-containing protein	-
K3F43_RS14470 (K3F43_14470)	3265023..3265409	+	387	WP_045182269.1	DUF2946 domain-containing protein	-
K3F43_RS14475 (K3F43_14475)	3265457..3265927	+	471	WP_101292298.1	copper chaperone PCu(A)C	-
K3F43_RS14480 (K3F43_14480)	3265947..3266375	+	429	WP_045182264.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15307.66 Da
• Isoelectric Point: 7.9271

>T212301 WP_045182275.1 NZ_CP080626:3262947-3263363 [Pseudomonas sp. S11A 273]
MLKYMLDTNICIFTLKNKPQAIREAFNRHHGQLCISTITLMELIYGAEKSAAAERNLAVVEGFAARLEVLSYDSHAAAHS
GQLRAELARAGTPIGPYDQMIAGHARSLGLLLVTNNMREFQRVPGLRVEDWLNSSVQL

Nucleotide

• Length: 417 bp

>T212301 NZ_CP107001:4032571-4032828 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.45 Da
• Isoelectric Point: 4.5702

>AT212301 WP_045182277.1 NZ_CP080626:3262716-3262946 [Pseudomonas sp. S11A 273]
MEQGAVFKSNRSQAVRLPKSVALPEDVKRVDVVAVGRTRIISPAGETWDSWFEGPSVSADFMAEREQPTDQEREGF

Nucleotide

• Length: 231 bp

>AT212301 NZ_CP107001:4032840-4033385 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA