Detailed information of TA system TA212300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3238229..3238881
• Replicon: chromosome
• Accession: NZ_CP080624
• Organism: Acidiferrobacter thiooxydans strain ZJ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A9R16_RS15090
• Protein ID: WP_083995821.1
• Coordinates: 3238468..3238881 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1C2G1V1
• Locus tag: A9R16_RS15085
• Protein ID: WP_065970385.1
• Coordinates: 3238229..3238471 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9R16_RS15065	3233560..3234510	+	951	Protein_2961	ISL3 family transposase	-
A9R16_RS15070 (A9R16_015240)	3234524..3235213	-	690	WP_228579759.1	RHS repeat-associated core domain-containing protein	-
A9R16_RS15075 (A9R16_015245)	3235693..3236577	-	885	WP_228579762.1	DUF4338 domain-containing protein	-
A9R16_RS15080 (A9R16_015250)	3236586..3237872	-	1287	WP_228579923.1	ISNCY family transposase	-
A9R16_RS15085 (A9R16_015255)	3238229..3238471	+	243	WP_065970385.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A9R16_RS15090 (A9R16_015260)	3238468..3238881	+	414	WP_083995821.1	type II toxin-antitoxin system VapC family toxin	Toxin
A9R16_RS15095 (A9R16_015265)	3238959..3239234	-	276	WP_065970380.1	hypothetical protein	-
A9R16_RS15100 (A9R16_015270)	3239358..3240008	+	651	WP_228579764.1	hypothetical protein	-
A9R16_RS15105 (A9R16_015275)	3240250..3242910	-	2661	WP_228579766.1	DNA polymerase I	-
A9R16_RS15110 (A9R16_015280)	3242909..3243661	+	753	WP_065969089.1	TIGR00730 family Rossman fold protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3222997..3248571	25574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-127)

Antitoxin

• SpoVT-AbrB domain (11-46)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14780.01 Da
• Isoelectric Point: 6.8612

>T212300 WP_083995821.1 NZ_CP080624:3238468-3238881 [Acidiferrobacter thiooxydans]
MSASLAYLLDTNTLSDLVRNPQGVVAAQITKAGEDTVCTSIVVAAELRYGATKSNSAKLAERIDLICSALEILPLEIPAD
HRYALVRHHLSRQGTPIGPNDLLIAAHALARGLTVVTANVREFSRVPELKVENWLQA

Nucleotide

• Length: 414 bp

>T212300 NZ_CP107001:3759975-3760253 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9169.47 Da
• Isoelectric Point: 4.5084

>AT212300 WP_065970385.1 NZ_CP080624:3238229-3238471 [Acidiferrobacter thiooxydans]
MSTSERHVRLFRNGRNQALRIPRELELEGEEALIYKEGDRLIVEPIHKGRLLALLASLDPLTEPFPNIDDDLPPLDDVQL

Nucleotide

• Length: 243 bp

>AT212300 NZ_CP107001:3759712-3759972 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1C2G1V1