Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 1621304..1621918 Replicon chromosome
Accession NZ_CP080624
Organism Acidiferrobacter thiooxydans strain ZJ

Toxin (Protein)


Gene name PumA Uniprot ID A0A1C2FX75
Locus tag A9R16_RS07725 Protein ID WP_065972400.1
Coordinates 1621304..1621600 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1C2FWZ2
Locus tag A9R16_RS07730 Protein ID WP_065972399.1
Coordinates 1621628..1621918 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9R16_RS07705 (A9R16_007780) 1617347..1618495 - 1149 WP_065972404.1 ATPase, T2SS/T4P/T4SS family -
A9R16_RS07710 (A9R16_007785) 1618492..1619349 - 858 WP_083996391.1 type IV secretory system conjugative DNA transfer family protein -
A9R16_RS07715 (A9R16_007790) 1619346..1619792 - 447 WP_065972402.1 DotD/TraH family lipoprotein -
A9R16_RS07720 (A9R16_007795) 1620295..1621227 + 933 WP_065972401.1 site-specific integrase -
A9R16_RS07725 (A9R16_007800) 1621304..1621600 + 297 WP_065972400.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A9R16_RS07730 (A9R16_007805) 1621628..1621918 + 291 WP_065972399.1 putative addiction module antidote protein Antitoxin
A9R16_RS07735 (A9R16_007810) 1622122..1622727 + 606 WP_065972398.1 hypothetical protein -
A9R16_RS07740 (A9R16_007815) 1622806..1623159 + 354 WP_065972397.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
A9R16_RS07745 (A9R16_007820) 1623156..1623743 + 588 WP_065972396.1 hypothetical protein -
A9R16_RS07750 (A9R16_007825) 1624254..1624484 + 231 WP_065972395.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1620295..1709453 89158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-85)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-92)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 10934.65 Da        Isoelectric Point: 11.3022

>T212297 WP_065972400.1 NZ_CP080624:1621304-1621600 [Acidiferrobacter thiooxydans]
VIEVRKTDVYVRWLDTLRDERARARVLARIERLLTGNAGDVRPVGGGVSELRIDYGPGYRVYFVRRGQALVILLAGGDKG
SQKADIRRALILARNLSS

Download         Length: 297 bp

>T212297 NZ_CP107001:3561455-3561673 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10290.74 Da        Isoelectric Point: 7.2766

>AT212297 WP_065972399.1 NZ_CP080624:1621628-1621918 [Acidiferrobacter thiooxydans]
MPKTKTSRYDVAEHLRTPKEMAAYLEACLEEADGDAGFIAKALGDIARAKGMSQVARDAGLSRESLYKALSGERSPDFST
ILKVVNALGISLHAHA

Download         Length: 291 bp

>AT212297 NZ_CP107001:3561055-3561429 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C2FX75

Antitoxin

Source ID Structure
AlphaFold DB A0A1C2FWZ2

References