Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4391709..4392388 Replicon chromosome
Accession NZ_CP080620
Organism Escherichia coli strain TOP10

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag K3F56_RS21190 Protein ID WP_000854672.1
Coordinates 4391709..4392050 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag K3F56_RS21195 Protein ID WP_000070395.1
Coordinates 4392071..4392388 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3F56_RS21165 (4387583) 4387583..4387961 - 379 Protein_4169 hypothetical protein -
K3F56_RS21170 (4388122) 4388122..4388481 - 360 Protein_4170 porin -
K3F56_RS21175 (4388769) 4388769..4389872 + 1104 WP_001285288.1 glutamate 5-kinase -
K3F56_RS21180 (4389884) 4389884..4391137 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
K3F56_RS21190 (4391709) 4391709..4392050 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
K3F56_RS21195 (4392071) 4392071..4392388 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
K3F56_RS21200 (4392407) 4392407..4392628 - 222 WP_087122211.1 DUF987 domain-containing protein -
K3F56_RS21205 (4392637) 4392637..4393113 - 477 WP_000811693.1 RadC family protein -
K3F56_RS21210 (4393129) 4393129..4393587 - 459 WP_000211838.1 antirestriction protein -
K3F56_RS21215 (4393685) 4393685..4393924 - 240 WP_000194654.1 DUF905 family protein -
K3F56_RS21220 (4394001) 4394001..4394468 - 468 WP_001547765.1 protein YkfB -
K3F56_RS21225 (4394491) 4394491..4394934 - 444 WP_000824223.1 lipoprotein YafY -
K3F56_RS21230 (4394934) 4394934..4395110 - 177 WP_001285112.1 hypothetical protein -
K3F56_RS21235 (4395157) 4395157..4395348 - 192 Protein_4182 DeoR family transcriptional regulator -
K3F56_RS21240 (4395565) 4395565..4396386 - 822 WP_000197389.1 DUF932 domain-containing protein -
K3F56_RS21245 (4396478) 4396478..4397341 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T212295 WP_000854672.1 NZ_CP080620:c4392050-4391709 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T212295 NZ_CP107001:2670509-2670616 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT212295 WP_000070395.1 NZ_CP080620:c4392388-4392071 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT212295 NZ_CP107001:c2670462-2670396 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References