Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4206624..4207455 Replicon chromosome
Accession NZ_CP080620
Organism Escherichia coli strain TOP10

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K3F56_RS20130 Protein ID WP_000854814.1
Coordinates 4207081..4207455 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag K3F56_RS20125 Protein ID WP_001285584.1
Coordinates 4206624..4206992 (+) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3F56_RS20110 (4204291) 4204291..4205823 + 1533 WP_001350525.1 protein YeeR -
K3F56_RS20115 (4205820) 4205820..4206266 + 447 WP_000187523.1 RadC family protein -
K3F56_RS20120 (4206329) 4206329..4206550 + 222 WP_000692323.1 DUF987 domain-containing protein -
K3F56_RS20125 (4206624) 4206624..4206992 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K3F56_RS20130 (4207081) 4207081..4207455 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K3F56_RS20135 (4207452) 4207452..4207646 + 195 WP_000988600.1 DUF5983 family protein -
K3F56_RS20140 (4207692) 4207692..4207772 + 81 Protein_3965 hypothetical protein -
K3F56_RS20145 (4208061) 4208061..4208189 - 129 Protein_3966 transposase domain-containing protein -
K3F56_RS20150 (4208309) 4208309..4208443 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
K3F56_RS20155 (4208544) 4208544..4208873 - 330 WP_000450409.1 DUF496 family protein -
K3F56_RS20160 (4209045) 4209045..4210103 - 1059 WP_046613327.1 FUSC family protein -
K3F56_RS20165 (4210301) 4210301..4210774 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
K3F56_RS20170 (4210893) 4210893..4212059 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T212290 WP_000854814.1 NZ_CP080620:4207081-4207455 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T212290 NZ_CP107001:c1812379-1812005 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT212290 WP_001285584.1 NZ_CP080620:4206624-4206992 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT212290 NZ_CP107001:c1812836-1812468 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829FY50


Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References