Detailed information of TA system TA212289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4108206..4109020
• Replicon: chromosome
• Accession: NZ_CP080620
• Organism: Escherichia coli strain TOP10

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K3F56_RS19625
• Protein ID: WP_001054376.1
• Coordinates: 4108206..4108463 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K3F56_RS19630
• Protein ID: WP_001309181.1
• Coordinates: 4108475..4109020 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3F56_RS19605 (4103494)	4103494..4104600	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
K3F56_RS19610 (4104665)	4104665..4105645	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K3F56_RS22145 (4105755)	4105755..4105960	+	206	Protein_3858	HNH endonuclease	-
K3F56_RS19615 (4106228)	4106228..4107468	-	1241	Protein_3859	helicase YjhR	-
K3F56_RS22150 (4107584)	4107584..4107715	+	132	WP_001309182.1	hypothetical protein	-
K3F56_RS19625 (4108206)	4108206..4108463	+	258	WP_001054376.1	YjhX family toxin	Toxin
K3F56_RS19630 (4108475)	4108475..4109020	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K3F56_RS19635 (4109076)	4109076..4109822	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K3F56_RS19640 (4109991)	4109991..4110209	+	219	Protein_3864	hypothetical protein	-
K3F56_RS19645 (4110247)	4110247..4110363	+	117	Protein_3865	VOC family protein	-
K3F56_RS19650 (4110608)	4110608..4111729	+	1122	WP_000010829.1	M42 family metallopeptidase	-
K3F56_RS19655 (4111726)	4111726..4112004	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K3F56_RS19660 (4112016)	4112016..4113329	+	1314	WP_000460842.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4100700..4117244	16544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T212289 WP_001054376.1 NZ_CP080620:4108206-4108463 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T212289 NZ_CP107001:1800483-1800737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT212289 WP_001309181.1 NZ_CP080620:4108475-4109020 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT212289 NZ_CP107001:1800235-1800486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8