Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2653285..2654722 Replicon chromosome
Accession NZ_CP080620
Organism Escherichia coli strain TOP10

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag K3F56_RS12615 Protein ID WP_000155570.1
Coordinates 2653285..2654358 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag K3F56_RS12620 Protein ID WP_000461704.1
Coordinates 2654351..2654722 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3F56_RS12600 (2648718) 2648718..2650907 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
K3F56_RS12605 (2650904) 2650904..2652520 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
K3F56_RS12610 (2652880) 2652880..2653143 - 264 WP_000502050.1 DUF1524 domain-containing protein -
K3F56_RS12615 (2653285) 2653285..2654358 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
K3F56_RS12620 (2654351) 2654351..2654722 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
K3F56_RS12625 (2655077) 2655077..2655940 + 864 WP_000787597.1 GTPase family protein -
K3F56_RS12630 (2656032) 2656032..2656853 + 822 WP_000197391.1 DUF932 domain-containing protein -
K3F56_RS12635 (2657070) 2657070..2657771 + 702 WP_000189409.1 WYL domain-containing protein -
K3F56_RS12640 (2657812) 2657812..2658048 + 237 WP_001144029.1 protein YpjK -
K3F56_RS12645 (2658048) 2658048..2658491 + 444 WP_000824222.1 lipoprotein YfjS -
K3F56_RS12650 (2658515) 2658515..2658982 + 468 WP_000702495.1 protein YfjT -
K3F56_RS22105 (2659369) 2659369..2659521 - 153 Protein_2491 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T212272 WP_000155570.1 NZ_CP080620:2653285-2654358 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T212272 NZ_CP107000:2671044-2671151 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT212272 WP_000461704.1 NZ_CP080620:2654351-2654722 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT212272 NZ_CP107000:c2670997-2670931 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References