Detailed information of TA system TA212270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1856025..1856643
• Replicon: chromosome
• Accession: NZ_CP080620
• Organism: Escherichia coli strain TOP10

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: K3F56_RS08800
• Protein ID: WP_001290581.1
• Coordinates: 1856025..1856243 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: K3F56_RS08805
• Protein ID: WP_000344800.1
• Coordinates: 1856269..1856643 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3F56_RS08765 (1851314)	1851314..1851886	+	573	WP_000779842.1	YbaY family lipoprotein	-
K3F56_RS08770 (1851917)	1851917..1852228	-	312	WP_000409911.1	MGMT family protein	-
K3F56_RS08780 (1852607)	1852607..1852960	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
K3F56_RS08785 (1853002)	1853002..1854552	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
K3F56_RS08790 (1854716)	1854716..1855186	-	471	WP_000136192.1	YlaC family protein	-
K3F56_RS08795 (1855302)	1855302..1855853	-	552	WP_000102564.1	maltose O-acetyltransferase	-
K3F56_RS08800 (1856025)	1856025..1856243	-	219	WP_001290581.1	HHA domain-containing protein	Toxin
K3F56_RS08805 (1856269)	1856269..1856643	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
K3F56_RS08810 (1857189)	1857189..1860338	-	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
K3F56_RS08815 (1860361)	1860361..1861554	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T212270 WP_001290581.1 NZ_CP080620:c1856243-1856025 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T212270 NZ_CP107000:c2382413-2382237 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT212270 WP_000344800.1 NZ_CP080620:c1856643-1856269 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT212270 NZ_CP107000:c2382191-2381775 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5