Detailed information of TA system TA021227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 834776..835305
• Replicon: chromosome
• Accession: NC_007350
• Organism: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q49Z22
• Locus tag: SSP_RS03995
• Protein ID: WP_011302703.1
• Coordinates: 834943..835305 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q49Z23
• Locus tag: SSP_RS03990
• Protein ID: WP_011302702.1
• Coordinates: 834776..834946 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSP_RS03965	830507..830986	+	480	WP_011302698.1	PH domain-containing protein	-
SSP_RS03970	830979..832517	+	1539	WP_041784781.1	PH domain-containing protein	-
SSP_RS03975	832510..833010	+	501	WP_011302700.1	PH domain-containing protein	-
SSP_RS03980	833074..833424	+	351	WP_002482750.1	holo-ACP synthase	-
SSP_RS03985	833540..834688	+	1149	WP_011302701.1	alanine racemase	-
SSP_RS03990	834776..834946	+	171	WP_011302702.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SSP_RS03995	834943..835305	+	363	WP_011302703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SSP_RS04000	835642..836643	+	1002	WP_011302704.1	PP2C family protein-serine/threonine phosphatase	-
SSP_RS04005	836722..837048	+	327	WP_011302705.1	anti-sigma factor antagonist	-
SSP_RS04010	837050..837532	+	483	WP_002482755.1	anti-sigma B factor RsbW	-
SSP_RS04020	837507..838272	+	766	Protein_797	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13482.70 Da
• Isoelectric Point: 10.3173

>T21227 WP_011302703.1 NC_007350:834943-835305 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSDKKMKEVNNAIDISLGLHTIRSHKA

Nucleotide

• Length: 363 bp

>T21227 NC_007350:834943-835305 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
ATGATGAGACGTGGTGACGTTTATTTAGCTGATTTATCACCAGTCCAGGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
AATTATACAAAACGATACTGGTAATAAATATAGTCCTACCGTAATTGTAGCCGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAATTAGATAAAGATTCTGTTATATTGCTAGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACTTATCTTTCTGATAAAAAAATGAAAGAAGTTAATAATGCCATTGA
TATTAGTTTAGGATTACATACTATTAGGAGTCACAAAGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6332.00 Da
• Isoelectric Point: 4.0121

>AT21227 WP_011302702.1 NC_007350:834776-834946 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
MASFNQSRIHNIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT21227 NC_007350:834776-834946 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
ATGGCAAGTTTCAACCAAAGTAGAATTCATAATATAGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGATTTGAA
CCTCTCCCTCGCAGCAGAAGCATTTCCGTTTGAATGTGAAGCTTGTGATTGCAACGAAGTATATATTAGCTCCAAGAATA
ATAATGAATGA

Structures

Toxin

• AlphaFold DB: Q49Z22

Antitoxin

• AlphaFold DB: Q49Z23