Detailed information of TA system TA212262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5315773..5316377
• Replicon: chromosome
• Accession: NZ_CP080597
• Organism: Pseudomonas sp. ERGC3:05

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K2E96_RS24645
• Protein ID: WP_064451579.1
• Coordinates: 5316189..5316377 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A172YZ10
• Locus tag: K2E96_RS24640
• Protein ID: WP_064451580.1
• Coordinates: 5315773..5316177 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2E96_RS24615 (K2E96_24235)	5310799..5311696	+	898	Protein_4842	urea transporter	-
K2E96_RS24620 (K2E96_24240)	5311706..5312530	+	825	WP_064451583.1	ion transporter	-
K2E96_RS24625 (K2E96_24245)	5312636..5313642	+	1007	Protein_4844	sulfate ABC transporter substrate-binding protein	-
K2E96_RS24630 (K2E96_24250)	5313777..5314391	-	615	WP_221294306.1	DUF5666 domain-containing protein	-
K2E96_RS24635 (K2E96_24255)	5314570..5315766	+	1197	WP_221294305.1	MFS transporter	-
K2E96_RS24640 (K2E96_24260)	5315773..5316177	-	405	WP_064451580.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K2E96_RS24645 (K2E96_24265)	5316189..5316377	-	189	WP_064451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
K2E96_RS24650 (K2E96_24270)	5316512..5317633	-	1122	WP_259762853.1	cytochrome P450	-
K2E96_RS24655 (K2E96_24275)	5317708..5318403	+	696	WP_221294303.1	helix-turn-helix transcriptional regulator	-
K2E96_RS24660	5318480..5318569	-	90	Protein_4851	phosphonate metabolism protein PhnP	-
K2E96_RS24665 (K2E96_24280)	5318582..5319154	-	573	WP_221294302.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
K2E96_RS24670 (K2E96_24285)	5319588..5319983	-	396	WP_221294301.1	hypothetical protein	-
K2E96_RS24675 (K2E96_24290)	5319999..5321183	-	1185	WP_221294300.1	c-type cytochrome biogenesis protein CcmI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7134.36 Da
• Isoelectric Point: 11.5760

>T212262 WP_064451579.1 NZ_CP080597:c5316377-5316189 [Pseudomonas sp. ERGC3:05]
VQSRLLIKALQEAGWVLDRVTGSHHIFTHRYSPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T212262 NZ_CP107000:c1111740-1111411 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14726.73 Da
• Isoelectric Point: 4.7437

>AT212262 WP_064451580.1 NZ_CP080597:c5316177-5315773 [Pseudomonas sp. ERGC3:05]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGVIPMPTSVAHHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT212262 NZ_CP107000:c1112078-1111761 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A172YZ10