Detailed information of TA system TA212241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 8304..8947
• Replicon: plasmid pCrono589-2
• Accession: NZ_CP080593
• Organism: Cronobacter sakazakii strain Crono-589

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K1721_RS21290
• Protein ID: WP_076738082.1
• Coordinates: 8531..8947 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: K1721_RS21285
• Protein ID: WP_076738083.1
• Coordinates: 8304..8534 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1721_RS21275 (K1721_21220)	3914..4273	-	360	WP_076738003.1	hypothetical protein	-
K1721_RS21280 (K1721_21225)	4836..7958	-	3123	WP_261001366.1	hypothetical protein	-
K1721_RS21285 (K1721_21230)	8304..8534	+	231	WP_076738083.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K1721_RS21290 (K1721_21235)	8531..8947	+	417	WP_076738082.1	type II toxin-antitoxin system VapC family toxin	Toxin
K1721_RS21295 (K1721_21240)	8962..9720	+	759	WP_254891487.1	hypothetical protein	-
K1721_RS21300 (K1721_21245)	10350..11516	+	1167	WP_261001367.1	hypothetical protein	-
K1721_RS21305 (K1721_21250)	11700..13553	-	1854	WP_200436551.1	asparagine synthase-related protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34303	34303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15154.59 Da
• Isoelectric Point: 7.2161

>T212241 WP_076738082.1 NZ_CP080593:8531-8947 [Cronobacter sakazakii]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSTITYSEMRFGATGPKASPRHVQLVEEFCARLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T212241 NZ_CP106999:1800483-1800737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8726.82 Da
• Isoelectric Point: 4.4563

>AT212241 WP_076738083.1 NZ_CP080593:8304-8534 [Cronobacter sakazakii]
MRTVSIFKNGNNRAIRLPRDMDFEGVSELEIVRDGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT212241 NZ_CP106999:1800235-1800486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA