Detailed information of TA system TA021224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1846094..1846580
• Replicon: chromosome
• Accession: NC_007296
• Organism: Streptococcus pyogenes MGAS6180

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q48QR0
• Locus tag: M28_RS09420
• Protein ID: WP_014407961.1
• Coordinates: 1846094..1846363 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A660A1J7
• Locus tag: M28_RS09425
• Protein ID: WP_014407962.1
• Coordinates: 1846353..1846580 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M28_RS09405	1841589..1842662	+	1074	WP_011285298.1	DUF4097 family beta strand repeat protein	-
M28_RS09410	1842779..1845052	-	2274	WP_021340697.1	YhgE/Pip domain-containing protein	-
M28_RS09415	1845187..1845720	+	534	WP_010922785.1	TetR/AcrR family transcriptional regulator	-
M28_RS09420	1846094..1846363	-	270	WP_014407961.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M28_RS09425	1846353..1846580	-	228	WP_014407962.1	hypothetical protein	Antitoxin
M28_RS09430	1846733..1847104	-	372	WP_011285302.1	DUF1492 domain-containing protein	-
M28_RS09435	1847094..1847441	-	348	WP_011285303.1	DUF1492 domain-containing protein	-
M28_RS09440	1847722..1847967	-	246	WP_011285304.1	hypothetical protein	-
M28_RS09445	1847942..1848217	-	276	WP_014407964.1	hypothetical protein	-
M28_RS09450	1848207..1848545	-	339	WP_030126557.1	replication protein	-
M28_RS09455	1848587..1848853	-	267	WP_011285307.1	hypothetical protein	-
M28_RS09460	1848850..1849638	-	789	WP_011285308.1	hypothetical protein	-
M28_RS09860	1849729..1849887	-	159	WP_011285309.1	hypothetical protein	-
M28_RS09465	1849901..1850146	-	246	WP_011285310.1	hypothetical protein	-
M28_RS09470	1850142..1850468	-	327	Protein_1761	hypothetical protein	-
M28_RS09865	1850470..1850643	-	174	WP_011285312.1	hypothetical protein	-
M28_RS09475	1850649..1850822	-	174	WP_000694580.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10497.50 Da
• Isoelectric Point: 10.5027

>T21224 WP_014407961.1 NC_007296:c1846363-1846094 [Streptococcus pyogenes MGAS6180]
MTYKLVVSDEVKKQLKKMDKHVGLMLAKDMKKRLDGLNNPRQFGKALTGQYKGLWRYRVGNYRVICDIVDNEMIILALEV
GHRKEIYKR

Nucleotide

• Length: 270 bp

>T21224 NC_007296:c1846363-1846094 [Streptococcus pyogenes MGAS6180]
ATGACTTATAAGCTAGTGGTGAGTGATGAAGTCAAGAAGCAACTCAAAAAGATGGATAAACATGTAGGGCTTATGTTAGC
CAAAGACATGAAGAAACGACTTGACGGGTTAAATAATCCTAGACAATTTGGAAAAGCCCTCACAGGTCAATATAAAGGAC
TTTGGCGCTATCGTGTTGGTAATTATCGGGTGATTTGTGACATTGTGGATAATGAAATGATTATCCTTGCTTTGGAAGTT
GGACACAGGAAAGAGATATACAAGCGCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8641.63 Da
• Isoelectric Point: 3.9607

>AT21224 WP_014407962.1 NC_007296:c1846580-1846353 [Streptococcus pyogenes MGAS6180]
MAMVSLRLNSAEEELFRSYATHTGKTLSELFKTALAQQIEDQLDYETGIQALKRFEENPITYSIDDLIEELENDL

Nucleotide

• Length: 228 bp

>AT21224 NC_007296:c1846580-1846353 [Streptococcus pyogenes MGAS6180]
ATGGCAATGGTATCATTAAGACTGAACAGCGCAGAGGAAGAACTTTTCAGAAGCTACGCTACACACACAGGCAAAACATT
ATCTGAATTATTCAAGACAGCACTAGCACAACAAATTGAAGATCAATTGGACTATGAAACAGGTATTCAAGCTTTAAAAC
GCTTTGAAGAAAATCCCATAACTTATTCTATCGATGATTTAATTGAGGAGTTGGAAAATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A660A202

Antitoxin

• AlphaFold DB: A0A660A1J7