Detailed information of TA system TA212238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3850981..3851651
• Replicon: chromosome
• Accession: NZ_CP080591
• Organism: Cronobacter sakazakii strain Crono-589

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A7V7RAE9
• Locus tag: K1721_RS18330
• Protein ID: WP_007891559.1
• Coordinates: 3851301..3851651 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: K1721_RS18325
• Protein ID: WP_014727946.1
• Coordinates: 3850981..3851304 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1721_RS18300 (K1721_18250)	3846289..3847422	-	1134	WP_032988096.1	slipin family protein	-
K1721_RS18305 (K1721_18255)	3847537..3847791	+	255	WP_032988097.1	hypothetical protein	-
K1721_RS18310 (K1721_18260)	3847898..3849490	+	1593	WP_032988098.1	RNA repair transcriptional activator RtcR	-
K1721_RS18315 (K1721_18265)	3849652..3850587	+	936	WP_004387518.1	ketopantoate/pantoate/pantothenate transporter PanS	-
K1721_RS18320 (K1721_18270)	3850678..3850950	+	273	WP_032988099.1	DUF3811 domain-containing protein	-
K1721_RS18325 (K1721_18275)	3850981..3851304	-	324	WP_014727946.1	helix-turn-helix transcriptional regulator	Antitoxin
K1721_RS18330 (K1721_18280)	3851301..3851651	-	351	WP_007891559.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K1721_RS18335 (K1721_18285)	3851868..3852173	+	306	WP_014727945.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K1721_RS18340 (K1721_18290)	3852178..3852453	+	276	WP_007852536.1	putative addiction module antidote protein	-
K1721_RS18345 (K1721_18295)	3852470..3854101	-	1632	WP_007891561.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3844258..3852453	8195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12982.04 Da
• Isoelectric Point: 10.0998

>T212238 WP_007891559.1 NZ_CP080591:c3851651-3851301 [Cronobacter sakazakii]
MIKPLFWVGQARKDLQALPEDVQDLFGYALYLAQQGRRHPQARPLKGFGGAGVLEVVEDYQGNAFRAVYTVCLKDAIYVL
HVFQKKSSSGIATPRPEMEKIEQRLKAAQRHAGGLK

Nucleotide

• Length: 351 bp

>T212238 NZ_CP106999:c1111740-1111411 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11794.88 Da
• Isoelectric Point: 7.9747

>AT212238 WP_014727946.1 NZ_CP080591:c3851304-3850981 [Cronobacter sakazakii]
MTADIERSNGNVYDDLGLIDAEEMQLKAQLAMAISDILKARGLTQQEAARLLGMTQPKLSLLLRGQFRGISETKMMACLM
LLGRDISIVVGEAQQARGKMRLIYQPA

Nucleotide

• Length: 324 bp

>AT212238 NZ_CP106999:c1112078-1111761 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A7V7RAE9