Detailed information of TA system TA021223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3873..4453
• Replicon: plasmid smallplasmid
• Accession: NC_007275
• Organism: Pseudomonas syringae pv. phaseolicola 1448A

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q48B35
• Locus tag: PSPPH_RS27180
• Protein ID: WP_011282511.1
• Coordinates: 4154..4453 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PSPPH_RS27175
• Protein ID: WP_003348777.1
• Coordinates: 3873..4157 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSPPH_RS27155	304..1617	+	1314	WP_011282525.1	replication initiation protein	-
PSPPH_RS27160	1757..2182	+	426	WP_011282542.1	S24 family peptidase	-
PSPPH_RS27165	2175..3458	+	1284	WP_011282506.1	Y-family DNA polymerase	-
PSPPH_RS27175	3873..4157	-	285	WP_003348777.1	putative addiction module antidote protein	Antitoxin
PSPPH_RS27180	4154..4453	-	300	WP_011282511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSPPH_RS27185	4625..4912	+	288	WP_041924941.1	hypothetical protein	-
PSPPH_RS27190	4964..5380	+	417	WP_011282521.1	hypothetical protein	-
PSPPH_RS27195	5774..6427	+	654	WP_003348786.1	AAA family ATPase	-
PSPPH_RS27200	6516..6767	+	252	WP_003348789.1	ribbon-helix-helix protein, CopG family	-
PSPPH_RS27205	7035..7676	+	642	WP_011282514.1	SOS response-associated peptidase	-
PSPPH_RS27210	8015..8518	+	504	WP_041924929.1	hypothetical protein	-
PSPPH_RS27215	8630..8932	+	303	WP_011282533.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..51711	51711

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11168.79 Da
• Isoelectric Point: 10.2231

>T21223 WP_011282511.1 NC_007275:c4453-4154 [Pseudomonas savastanoi pv. phaseolicola 1448A]
MTTIKQTSTYMAWERRLKDQKAKAAIAARIFRVANGLMGDVSPVGQGVSELRIHVGPGYRVYFQQRGDELILLLCGGDKS
SQSRDIETAKRLADQWRQE

Nucleotide

• Length: 300 bp

>T21223 NC_007275:c4453-4154 [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGACGACGATAAAACAGACATCTACCTACATGGCTTGGGAACGCAGGCTCAAAGACCAAAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCGCCAGTAGGGCAGGGCGTGAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAATTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCAAAGAGACTGGCTGATCAATGGAGGCAAGAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10263.80 Da
• Isoelectric Point: 5.0916

>AT21223 WP_003348777.1 NC_007275:c4157-3873 [Pseudomonas savastanoi pv. phaseolicola 1448A]
MKEQIFDYDPAAALDGPEAIAIFISDAFETGDAEYIAKAMGVVARAKGMSELARETGLSREQLYRSFSSKGNPTLKTMLT
VMRALGVDMTARMH

Nucleotide

• Length: 285 bp

>AT21223 NC_007275:c4157-3873 [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGGCCTGAGGCAATCGCTATTTTTATCTCTGACGC
ATTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGAGCGAGCTTGCGC
GTGAGACAGGTCTTTCTCGAGAGCAGCTTTACAGATCGTTCAGTAGCAAGGGCAACCCAACCTTAAAAACGATGTTGACG
GTAATGAGAGCCCTGGGCGTGGACATGACAGCTCGAATGCACTGA

Structures

Toxin

• AlphaFold DB: A0A3M3YY44