Detailed information of TA system TA021222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 126280..126827
• Replicon: plasmid largeplasmid
• Accession: NC_007274
• Organism: Pseudomonas syringae pv. phaseolicola 1448A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q48B48
• Locus tag: PSPPH_RS27110
• Protein ID: WP_003319696.1
• Coordinates: 126280..126606 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: E7PGZ6
• Locus tag: PSPPH_RS27115
• Protein ID: WP_004643921.1
• Coordinates: 126603..126827 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSPPH_RS27075	121721..122140	-	420	WP_004661570.1	type II toxin-antitoxin system VapC family toxin	-
PSPPH_RS27080	122137..122394	-	258	WP_003381346.1	Arc family DNA-binding protein	-
PSPPH_RS27085	122859..123563	+	705	WP_004661990.1	ParA family protein	-
PSPPH_RS27090	123560..123883	+	324	WP_004666942.1	hypothetical protein	-
PSPPH_RS29790	123994..124353	+	360	Protein_132	SOS response-associated peptidase family protein	-
PSPPH_RS27095	124306..124968	-	663	Protein_133	IS110 family transposase	-
PSPPH_RS27100	125043..125942	+	900	Protein_134	IS3-like element ISPsy24 family transposase	-
PSPPH_RS27105	125953..126162	-	210	Protein_135	hypothetical protein	-
PSPPH_RS27110	126280..126606	-	327	WP_003319696.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PSPPH_RS27115	126603..126827	-	225	WP_004643921.1	antitoxin MazE family protein	Antitoxin
PSPPH_RS27120	127165..127586	+	422	Protein_138	transposase	-
PSPPH_RS27125	127806..129581	+	1776	Protein_139	conjugal transfer protein	-
PSPPH_RS27135	129821..130069	+	249	Protein_140	type IV secretion system protein	-
PSPPH_RS27140	130059..130592	+	534	Protein_141	TrbG/VirB9 family P-type conjugative transfer protein	-
PSPPH_RS27145	130713..131363	+	651	WP_005730296.1	AAA family ATPase	-
PSPPH_RS27150	131354..131707	+	354	WP_005730297.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131950	131950
	-	inside	IScluster/Tn	-	-	125028..127443	2415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (8-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11660.71 Da
• Isoelectric Point: 8.0546

>T21222 WP_003319696.1 NC_007274:c126606-126280 [Pseudomonas savastanoi pv. phaseolicola 1448A]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Nucleotide

• Length: 327 bp

>T21222 NC_007274:c126606-126280 [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTTAGCGAACACACCAGCGTTACCGTGCTTCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACCCTCAAGCGTGAAAAA
ATAGGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8501.64 Da
• Isoelectric Point: 6.2008

>AT21222 WP_004643921.1 NC_007274:c126827-126603 [Pseudomonas savastanoi pv. phaseolicola 1448A]
MPTSHVNTRVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Nucleotide

• Length: 225 bp

>AT21222 NC_007274:c126827-126603 [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGCCTACATCCCATGTAAATACCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Structures

Toxin

• AlphaFold DB: A0A7V8S2J7

Antitoxin

• AlphaFold DB: A0A0P9S763