Detailed information of TA system TA212215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/-
• Location: 645261..646840
• Replicon: chromosome
• Accession: NZ_CP080589
• Organism: Xanthomonas oryzae pv. oryzicola strain GX01

Toxin (Protein)

• Gene name: avrRxo1
• Uniprot ID: -
• Locus tag: K2I41_RS03190
• Protein ID: WP_149621311.1
• Coordinates: 645261..646526 (+)
• Length: 422 a.a.

Antitoxin (Protein)

• Gene name: arc1
• Uniprot ID: A0A0C5UZA4
• Locus tag: K2I41_RS03195
• Protein ID: WP_044749828.1
• Coordinates: 646544..646840 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2I41_RS03175 (K2I41_03180)	641254..641619	-	366	WP_224229821.1	SymE family type I addiction module toxin	-
K2I41_RS03180	643331..644356	+	1026	Protein_550	hemagglutinin repeat-containing protein	-
K2I41_RS03185 (K2I41_03190)	644356..644886	+	531	Protein_551	IS30 family transposase	-
K2I41_RS03190 (K2I41_03195)	645261..646526	+	1266	WP_149621311.1	zeta toxin family type III secretion system effector XopAJ/AvrRxo1	Toxin
K2I41_RS03195 (K2I41_03200)	646544..646840	+	297	WP_044749828.1	hypothetical protein	Antitoxin
K2I41_RS03200 (K2I41_03205)	647062..647328	+	267	Protein_554	transposase	-
K2I41_RS03205 (K2I41_03210)	647395..647820	-	426	Protein_555	integrase core domain-containing protein	-
K2I41_RS03210 (K2I41_03215)	647949..648990	+	1042	WP_229690555.1	IS3 family transposase	-
K2I41_RS03215 (K2I41_03220)	649020..649097	+	78	Protein_557	anti-sigma factor antagonist	-
K2I41_RS03220 (K2I41_03225)	649192..649367	+	176	Protein_558	HAD family hydrolase	-
K2I41_RS03225 (K2I41_03230)	649583..650335	-	753	WP_024712407.1	YceI family protein	-
K2I41_RS03230 (K2I41_03235)	650546..651136	-	591	WP_014504809.1	malonic semialdehyde reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	640811..656771	15960
	-	inside	IScluster/Tn	-	-	633634..648990	15356

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (149-291)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 422 a.a.
• Molecular weight: 45201.81 Da
• Isoelectric Point: 6.9140

>T212215 WP_149621311.1 NZ_CP080589:645261-646526 [Xanthomonas oryzae pv. oryzicola]
MKNKTDIAGIAGLSTSHVSFDDAESKSVLDGSTQSTPEYKFQTPLDQLPARPAGPKSSNTTDAYIHESLPHSVPIAGAST
SAVTHQQATQRKGTDEIYGLGSLPSAGPERWDYLANPGNWHPERRKLHEKLLDQARSSALTLAESLESDGCQPTLFALRG
NTATGKTRIATKKIPVLAAALKKTGGKGCVNPDVFKSSLAKSEAGAKIFSSAQVHSESSFLADRFEGGLRSQKTGSGAIA
SIVVDKRLSRAYEIDSYIQLAKETGRKVELCDIDAPLENSLVGVLQRKPEGEDPRPPYPVVSSGFVAVRSNRMYVIDRFI
ADPSLGNYRLFGTAEDGEKVMVATVIGGEFSVENAELYEKITSPQLSVTGDLADKVIDKELIDRLKNNIADPERAAKTQA
ALEKYSGKSWSAALAAHSELI

Nucleotide

• Length: 1266 bp

>T212215 NZ_CP106998:c1123275-1122202 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11327.83 Da
• Isoelectric Point: 5.2138

>AT212215 WP_044749828.1 NZ_CP080589:646544-646840 [Xanthomonas oryzae pv. oryzicola]
MKTLTGADALEFHKKLKERNKALHASDLELALVHADAVGKERFDLEELEKICDTSDAGRLTDAKERHDIYERMYYVEYPN
VMTLKEFAHIVETLSSWS

Nucleotide

• Length: 297 bp

>AT212215 NZ_CP106998:c1122209-1121838 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0C5UZA4