Detailed information of TA system TA212213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 8983..9476
• Replicon: plasmid pXOCgx01
• Accession: NZ_CP080588
• Organism: Xanthomonas oryzae pv. oryzicola strain GX01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K2I41_RS00045
• Protein ID: WP_193566048.1
• Coordinates: 8983..9309 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K2I41_RS00050
• Protein ID: WP_229697988.1
• Coordinates: 9306..9476 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2I41_RS00035 (K2I41_00035)	4366..5940	+	1575	WP_150411549.1	type IV secretion system DNA-binding domain-containing protein	-
K2I41_RS00040 (K2I41_00040)	5954..8914	+	2961	WP_048485071.1	MobF family relaxase	-
K2I41_RS00045 (K2I41_00045)	8983..9309	-	327	WP_193566048.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K2I41_RS00050 (K2I41_00050)	9306..9476	-	171	WP_229697988.1	antitoxin MazE family protein	Antitoxin
K2I41_RS00055 (K2I41_00055)	9743..10162	-	420	WP_048485073.1	hypothetical protein	-
K2I41_RS00060 (K2I41_00060)	10288..11196	-	909	WP_048485074.1	lytic transglycosylase domain-containing protein	-
K2I41_RS00065 (K2I41_00065)	11183..12205	-	1023	WP_048485075.1	P-type DNA transfer ATPase VirB11	-
K2I41_RS00070 (K2I41_00070)	12216..13436	-	1221	WP_048485076.1	TrbI/VirB10 family protein	-
K2I41_RS00075 (K2I41_00075)	13438..14220	-	783	WP_075244577.1	P-type conjugative transfer protein VirB9	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53205	53205

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (2-54)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11646.72 Da
• Isoelectric Point: 10.1008

>T212213 WP_193566048.1 NZ_CP080588:c9309-8983 [Xanthomonas oryzae pv. oryzicola]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVVIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T212213 NZ_CP106998:977325-977660 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6460.31 Da
• Isoelectric Point: 4.2325

>AT212213 WP_229697988.1 NZ_CP080588:c9476-9306 [Xanthomonas oryzae pv. oryzicola]
MAGLRPVQIWVPDTRRPDFAAECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 171 bp

>AT212213 NZ_CP106998:977077-977325 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA