Detailed information of TA system TA212192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 248743..249380
• Replicon: chromosome
• Accession: NZ_CP080573
• Organism: Stenotrophomonas maltophilia strain FZD2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: K1516_RS01095
• Protein ID: WP_153856750.1
• Coordinates: 249090..249380 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: M5CST9
• Locus tag: K1516_RS01090
• Protein ID: WP_004136660.1
• Coordinates: 248743..249051 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1516_RS01070 (K1516_01065)	244404..245294	+	891	WP_248858708.1	3-hydroxyisobutyrate dehydrogenase	-
K1516_RS01075 (K1516_01070)	245505..245930	-	426	WP_014035621.1	organic hydroperoxide resistance protein	-
K1516_RS01080 (K1516_01075)	246042..246998	-	957	WP_049399847.1	cation transporter	-
K1516_RS01085 (K1516_01080)	247231..248685	+	1455	WP_248858710.1	SulP family inorganic anion transporter	-
K1516_RS01090 (K1516_01085)	248743..249051	-	309	WP_004136660.1	putative addiction module antidote protein	Antitoxin
K1516_RS01095 (K1516_01090)	249090..249380	-	291	WP_153856750.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K1516_RS01100 (K1516_01095)	249628..250659	+	1032	WP_180854538.1	tRNA dihydrouridine(20/20a) synthase DusA	-
K1516_RS01105 (K1516_01100)	250873..251247	+	375	WP_110710766.1	hypothetical protein	-
K1516_RS01110 (K1516_01105)	251321..252004	+	684	WP_004136763.1	response regulator transcription factor	-
K1516_RS01115 (K1516_01110)	252030..253454	+	1425	WP_094001618.1	sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	248743..261317	12574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10782.35 Da
• Isoelectric Point: 11.9277

>T212192 WP_153856750.1 NZ_CP080573:c249380-249090 [Stenotrophomonas maltophilia]
MATLQATETFTRWLRGLNNSGTRAVIVERLQRVARGLEGDVRSVGHGVSELRINHGPGYRVYFTRRGRSIVVLLHGGDKS
SQRRDIEKAVRLAELL

Nucleotide

• Length: 291 bp

>T212192 NZ_CP106997:1800235-1800486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10662.10 Da
• Isoelectric Point: 6.2132

>AT212192 WP_004136660.1 NZ_CP080573:c249051-248743 [Stenotrophomonas maltophilia]
MSKLKTIPFDPADYLDDDVAIATYLSEALAAGDAGHFQEALGTVARARGMGGVAEVSGLGRESLYKALRPGAHPRFDTVQ
RVLSALGVHLIVQADKQPAGTA

Nucleotide

• Length: 309 bp

>AT212192 NZ_CP106997:1800483-1800737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: M5CST9