Detailed information of TA system TA212183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2227328..2227857
• Replicon: chromosome
• Accession: NZ_CP080566
• Organism: Staphylococcus aureus strain HL20835 isolate HL20835

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMO16_RS11270
• Protein ID: WP_000621175.1
• Coordinates: 2227328..2227690 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMO16_RS11275
• Protein ID: WP_000948331.1
• Coordinates: 2227687..2227857 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO16_RS11250 (2224306)	2224306..2225076	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
JMO16_RS11255 (2225051)	2225051..2225530	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JMO16_RS11260 (2225532)	2225532..2225858	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMO16_RS11265 (2225977)	2225977..2226978	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JMO16_RS11270 (2227328)	2227328..2227690	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMO16_RS11275 (2227687)	2227687..2227857	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMO16_RS11280 (2227942)	2227942..2229090	-	1149	WP_001281154.1	alanine racemase	-
JMO16_RS11285 (2229156)	2229156..2229515	-	360	WP_000581197.1	holo-ACP synthase	-
JMO16_RS11290 (2229519)	2229519..2230010	-	492	WP_001205912.1	PH domain-containing protein	-
JMO16_RS11295 (2229997)	2229997..2231580	-	1584	WP_001294626.1	PH domain-containing protein	-
JMO16_RS11300 (2231573)	2231573..2232052	-	480	WP_001287087.1	hypothetical protein	-
JMO16_RS11305 (2232260)	2232260..2232820	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212183 WP_000621175.1 NZ_CP080566:c2227690-2227328 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212183 NZ_CP106996:c4215162-4214989 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212183 WP_000948331.1 NZ_CP080566:c2227857-2227687 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212183 NZ_CP106996:c4215486-4215190 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1