Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 29350..29924 Replicon plasmid largeplasmid
Accession NC_007274
Organism Pseudomonas syringae pv. phaseolicola 1448A

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag PSPPH_RS26635 Protein ID WP_003344395.1
Coordinates 29350..29727 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48BC4
Locus tag PSPPH_RS26640 Protein ID WP_004644067.1
Coordinates 29724..29924 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSPPH_RS26615 25063..25578 + 516 Protein_28 IS110-like element ISPsy16 family transposase -
PSPPH_RS26620 26340..28610 + 2271 WP_032704248.1 hypothetical protein -
PSPPH_RS30235 28613..28783 - 171 Protein_30 transposase -
PSPPH_RS26630 29016..29360 + 345 Protein_31 conjugal transfer protein -
PSPPH_RS26635 29350..29727 - 378 WP_003344395.1 PIN domain-containing protein Toxin
PSPPH_RS26640 29724..29924 - 201 WP_004644067.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
PSPPH_RS26645 30016..30696 + 681 Protein_34 hypothetical protein -
PSPPH_RS26650 30683..30952 + 270 Protein_35 type IV secretory system conjugative DNA transfer family protein -
PSPPH_RS26655 31210..32442 - 1233 Protein_36 IS91 family transposase -
PSPPH_RS26660 32654..32884 + 231 WP_019331426.1 hypothetical protein -
PSPPH_RS26665 32974..33513 + 540 WP_050772298.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..131950 131950
- flank IS/Tn - - 25081..25578 497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T21218 WP_003344395.1 NC_007274:c29727-29350 [Pseudomonas savastanoi pv. phaseolicola 1448A]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T21218 NC_007274:c29727-29350 [Pseudomonas savastanoi pv. phaseolicola 1448A]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCCAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATAGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGACTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7445.66 Da        Isoelectric Point: 7.0102

>AT21218 WP_004644067.1 NC_007274:c29924-29724 [Pseudomonas savastanoi pv. phaseolicola 1448A]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPTMEDIARRHEKAL

Download         Length: 201 bp

>AT21218 NC_007274:c29924-29724 [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGACAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAA
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4IKB9

References