Detailed information of TA system TA212161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2081752..2082281
• Replicon: chromosome
• Accession: NZ_CP080564
• Organism: Staphylococcus aureus strain HL16278

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMO07_RS10195
• Protein ID: WP_000621175.1
• Coordinates: 2081752..2082114 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMO07_RS10200
• Protein ID: WP_000948331.1
• Coordinates: 2082111..2082281 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO07_RS10175 (2078731)	2078731..2079501	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
JMO07_RS10180 (2079476)	2079476..2079955	-	480	WP_088867611.1	anti-sigma B factor RsbW	-
JMO07_RS10185 (2079957)	2079957..2080283	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMO07_RS10190 (2080402)	2080402..2081403	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JMO07_RS10195 (2081752)	2081752..2082114	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMO07_RS10200 (2082111)	2082111..2082281	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMO07_RS10205 (2082366)	2082366..2083514	-	1149	WP_001281142.1	alanine racemase	-
JMO07_RS10210 (2083580)	2083580..2083939	-	360	WP_000581197.1	holo-ACP synthase	-
JMO07_RS10215 (2083943)	2083943..2084434	-	492	WP_001205910.1	PH domain-containing protein	-
JMO07_RS10220 (2084421)	2084421..2086004	-	1584	WP_001294626.1	PH domain-containing protein	-
JMO07_RS10225 (2085997)	2085997..2086476	-	480	WP_001287090.1	hypothetical protein	-
JMO07_RS10230 (2086685)	2086685..2087245	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212161 WP_000621175.1 NZ_CP080564:c2082114-2081752 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212161 NZ_CP106996:c605876-605412 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212161 WP_000948331.1 NZ_CP080564:c2082281-2082111 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212161 NZ_CP106996:c606211-605876 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1