Detailed information of TA system TA021215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 2019340..2020327
• Replicon: chromosome
• Accession: NC_007181
• Organism: Sulfolobus acidocaldarius DSM 639

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IU00
• Locus tag: SACI_RS10550
• Protein ID: WP_011278971.1
• Coordinates: 2019340..2019957 (-)
• Length: 206 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I835
• Locus tag: SACI_RS10555
• Protein ID: WP_011278972.1
• Coordinates: 2019983..2020327 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACI_RS10535 (Saci_2180)	2014943..2015545	+	603	WP_011278968.1	hypothetical protein	-
SACI_RS10540 (Saci_2181)	2015597..2016988	-	1392	WP_011278969.1	APC family permease	-
SACI_RS10545 (Saci_2182)	2017139..2019133	+	1995	WP_011278970.1	primary-amine oxidase	-
SACI_RS10550 (Saci_2183)	2019340..2019957	-	618	WP_011278971.1	helix-turn-helix domain-containing protein	Toxin
SACI_RS10555 (Saci_2184)	2019983..2020327	-	345	WP_011278972.1	DUF488 domain-containing protein	Antitoxin
SACI_RS10560 (Saci_2185)	2020633..2021421	+	789	WP_011278973.1	aldose 1-epimerase	-
SACI_RS10565 (Saci_2186)	2021719..2022729	+	1011	WP_011278974.1	NAD-binding protein	-
SACI_RS10570 (Saci_2187)	2023317..2023946	-	630	WP_011278975.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (146-196)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 206 a.a.
• Molecular weight: 23779.04 Da
• Isoelectric Point: 9.9689

>T21215 WP_011278971.1 NC_007181:c2019957-2019340 [Sulfolobus acidocaldarius DSM 639]
MVFASKRPIEVTLLVDKHPCEVLDIFRKYEIKAQITNVKLRDQVTDHIAFLKINDDILKELKRRSLKTLRINENTIWIRT
NGCGVCKLLYHSDLIVEKVKVLSKATVMYKLLLPNLSALKQFLEELNSIRVEATVANVSEIDESELTDRQLEILRLAYKS
GYFDVDRKISMKELANKLGIKASTLEEILRRALKKAVKYYLDRKS

Nucleotide

• Length: 618 bp

>T21215 NC_007181:c2019957-2019340 [Sulfolobus acidocaldarius DSM 639]
ATGGTCTTTGCCTCAAAGAGACCCATTGAAGTTACACTCCTAGTGGATAAACACCCATGCGAAGTCTTAGACATATTTAG
AAAATATGAAATTAAGGCTCAGATAACTAATGTTAAGCTAAGGGATCAGGTTACTGACCATATTGCATTCCTTAAGATAA
ACGATGATATACTCAAGGAATTAAAGAGAAGGTCTCTTAAAACTTTAAGGATAAATGAAAATACCATTTGGATAAGGACA
AACGGTTGCGGCGTTTGTAAACTACTCTATCACTCTGACCTAATTGTGGAAAAGGTAAAGGTGTTAAGTAAAGCTACTGT
TATGTATAAACTTCTATTACCAAATCTTTCTGCATTGAAACAGTTTTTAGAAGAACTGAACAGCATAAGAGTAGAGGCTA
CAGTTGCCAATGTATCAGAAATTGACGAGAGCGAGTTAACAGATAGACAATTGGAAATCCTGAGATTGGCTTACAAATCA
GGCTATTTTGATGTCGATAGGAAAATCTCGATGAAGGAATTAGCCAATAAATTGGGGATAAAGGCATCTACGCTAGAGGA
GATACTGAGGAGGGCTCTAAAAAAGGCTGTAAAATATTATTTGGACAGAAAGAGCTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13706.92 Da
• Isoelectric Point: 9.2564

>AT21215 WP_011278972.1 NC_007181:c2020327-2019983 [Sulfolobus acidocaldarius DSM 639]
MIKVKRVYDAPESDDGVRILIDRLWPRGVKKDLVDIWMKDIAPSDELRKWFNHEPEKWEEFKHKYMEELRSNPKVKVLIE
VIRRSENVTFLYATKSPYNNAVVLKEYIESLLTK

Nucleotide

• Length: 345 bp

>AT21215 NC_007181:c2020327-2019983 [Sulfolobus acidocaldarius DSM 639]
ATGATTAAGGTCAAGCGAGTTTATGACGCACCTGAATCTGATGATGGGGTCAGAATATTGATTGACCGATTATGGCCTAG
AGGAGTAAAGAAAGATCTAGTGGATATTTGGATGAAAGATATTGCTCCATCAGATGAGTTAAGGAAATGGTTTAATCATG
AACCTGAAAAGTGGGAAGAGTTTAAGCACAAGTATATGGAGGAGCTTAGAAGTAACCCTAAGGTCAAAGTCTTAATCGAG
GTAATAAGGAGAAGCGAAAATGTTACATTCCTATATGCCACTAAATCGCCTTACAATAATGCGGTAGTTTTAAAGGAGTA
TATTGAAAGTTTGTTAACAAAATAA

Structures

Toxin

• AlphaFold DB: M1IU00

Antitoxin

• AlphaFold DB: M1I835