Detailed information of TA system TA021214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 2000353..2001335
• Replicon: chromosome
• Accession: NC_007181
• Organism: Sulfolobus acidocaldarius DSM 639

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1IXW8
• Locus tag: SACI_RS10480
• Protein ID: WP_011278959.1
• Coordinates: 2000715..2001335 (+)
• Length: 207 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I813
• Locus tag: SACI_RS10475
• Protein ID: WP_011278958.1
• Coordinates: 2000353..2000703 (+)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACI_RS10455 (Saci_2162)	1995757..1997691	-	1935	WP_011278954.1	DUF2079 domain-containing protein	-
SACI_RS10460 (Saci_2163)	1997900..1998604	+	705	WP_011278955.1	zinc ribbon domain-containing protein	-
SACI_RS10465 (Saci_2164)	1998688..1999332	-	645	WP_011278956.1	2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase	-
SACI_RS10470 (Saci_2165)	1999379..2000074	-	696	WP_011278957.1	glycerophosphodiester phosphodiesterase	-
SACI_RS10475 (Saci_2166)	2000353..2000703	+	351	WP_011278958.1	DUF488 family protein	Antitoxin
SACI_RS10480 (Saci_2167)	2000715..2001335	+	621	WP_011278959.1	helix-turn-helix domain-containing protein	Toxin
SACI_RS10485 (Saci_2168)	2001440..2002825	-	1386	WP_011278960.1	amidase	-
SACI_RS10490 (Saci_2169)	2003051..2003500	+	450	WP_230937910.1	hypothetical protein	-
SACI_RS10495 (Saci_2170)	2003590..2005236	-	1647	WP_011278962.1	thermopsin family protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (147-197)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 207 a.a.
• Molecular weight: 23490.89 Da
• Isoelectric Point: 9.6857

>T21214 WP_011278959.1 NC_007181:2000715-2001335 [Sulfolobus acidocaldarius DSM 639]
MGAGDVKKPINVTLILQNHPCLVMKLFEEMNVKAEINNVKMRESVTDHIATLKLTEKLLKELKERRSKTLRISENSVWIR
TEGCQVCKILYVSDAVVEKVKVVGSDAVMYKLIVPNLLSLKSLIDELNKIGVKAMVGSISELDEEQLTERQLEILKLSYK
MGFFDVDRRITMTELAEKLGIKAPTLEEILRRALRKAVKYYLDKKG

Nucleotide

• Length: 621 bp

>T21214 NC_007181:2000715-2001335 [Sulfolobus acidocaldarius DSM 639]
ATGGGTGCTGGTGATGTAAAGAAACCTATAAATGTTACACTTATTCTACAGAATCACCCCTGCTTAGTTATGAAATTATT
TGAAGAGATGAATGTAAAGGCGGAAATTAACAATGTGAAGATGAGGGAAAGTGTTACTGACCACATTGCAACCTTAAAGT
TAACTGAAAAGTTGCTTAAGGAGTTGAAAGAAAGAAGGTCAAAAACCTTGAGGATAAGTGAAAATAGCGTCTGGATCAGG
ACTGAAGGTTGCCAGGTATGTAAAATCCTTTATGTAAGTGACGCTGTAGTTGAGAAAGTGAAAGTTGTAGGAAGTGATGC
AGTTATGTATAAACTAATTGTTCCAAATCTACTCTCTCTGAAGAGTCTTATAGATGAGCTCAACAAGATAGGTGTAAAGG
CTATGGTAGGTAGCATATCTGAGCTAGATGAAGAGCAACTGACAGAAAGGCAATTGGAGATATTGAAATTATCATATAAG
ATGGGTTTCTTTGATGTAGACAGAAGAATAACCATGACGGAATTAGCTGAAAAACTTGGGATCAAGGCTCCTACTTTGGA
AGAGATACTGAGAAGGGCTCTACGAAAGGCAGTTAAGTATTATCTAGATAAAAAAGGATAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13918.19 Da
• Isoelectric Point: 8.2649

>AT21214 WP_011278958.1 NC_007181:2000353-2000703 [Sulfolobus acidocaldarius DSM 639]
MIKIKNVYDKPEINDGIRILVERTWPASVTRGSIDMWLRDLAPSEELITWYNYEPQKWEEFKRRYLEELRYNPKVKPLLM
IVKMTKIVTFLYSGDPTYNNAVVLKEYIESLINKES

Nucleotide

• Length: 351 bp

>AT21214 NC_007181:2000353-2000703 [Sulfolobus acidocaldarius DSM 639]
ATGATAAAAATAAAAAACGTTTATGACAAACCAGAGATTAACGACGGGATTAGAATATTGGTCGAGAGGACATGGCCAGC
CTCTGTAACTAGAGGCTCTATCGATATGTGGCTTAGGGATCTTGCCCCTTCTGAAGAATTAATAACATGGTATAATTATG
AACCGCAAAAATGGGAGGAATTTAAGAGAAGATATCTAGAAGAGTTGAGGTACAATCCAAAAGTTAAACCTTTATTGATG
ATAGTTAAGATGACTAAGATCGTAACATTTCTATACTCAGGAGACCCCACATACAATAACGCAGTTGTGCTCAAAGAATA
CATAGAAAGTTTAATAAATAAGGAAAGTTAA

Structures

Toxin

• AlphaFold DB: M1IXW8

Antitoxin

• AlphaFold DB: M1I813