Detailed information of TA system TA212135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 878284..878761
• Replicon: chromosome
• Accession: NZ_CP080562
• Organism: Staphylococcus aureus strain HL21008

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: JMO08_RS04135
• Protein ID: WP_000052076.1
• Coordinates: 878284..878472 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: JMO08_RS04140
• Protein ID: WP_001059690.1
• Coordinates: 878501..878761 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO08_RS04105 (JMO08_000821)	874278..874799	+	522	WP_000824482.1	hypothetical protein	-
JMO08_RS04110 (JMO08_000822)	875140..875826	+	687	WP_000141557.1	thermonuclease family protein	-
JMO08_RS04115 (JMO08_000823)	876183..876383	+	201	WP_001059082.1	cold-shock protein	-
JMO08_RS04120 (JMO08_000824)	876875..877093	-	219	WP_000005886.1	hypothetical protein	-
JMO08_RS04125 (JMO08_000825)	877155..877439	-	285	WP_044423470.1	hypothetical protein	-
JMO08_RS04130 (JMO08_000826)	877527..878096	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
JMO08_RS04135 (JMO08_000827)	878284..878472	-	189	WP_000052076.1	hypothetical protein	Toxin
JMO08_RS04140 (JMO08_000828)	878501..878761	-	261	WP_001059690.1	hypothetical protein	Antitoxin
JMO08_RS04145 (JMO08_000829)	879102..879305	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
JMO08_RS04150 (JMO08_000830)	879302..879538	-	237	WP_063456452.1	hypothetical protein	-
JMO08_RS04155 (JMO08_000831)	879763..880344	+	582	WP_001140693.1	histidine phosphatase family protein	-
JMO08_RS04160 (JMO08_000832)	880417..881034	-	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
JMO08_RS04165 (JMO08_000833)	881189..881683	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
JMO08_RS04170 (JMO08_000834)	882082..882504	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
JMO08_RS04175 (JMO08_000835)	882652..883368	+	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	clpP / vWbp	829214..998451	169237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T212135 WP_000052076.1 NZ_CP080562:c878472-878284 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T212135 NZ_CP106994:c4215162-4214989 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT212135 WP_001059690.1 NZ_CP080562:c878761-878501 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT212135 NZ_CP106994:c4215486-4215190 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56