Detailed information of TA system TA021212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 1766204..1766810
• Replicon: chromosome
• Accession: NC_007181
• Organism: Sulfolobus acidocaldarius DSM 639

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M1ITB5
• Locus tag: SACI_RS09440
• Protein ID: WP_011278759.1
• Coordinates: 1766204..1766599 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M1I7B9
• Locus tag: SACI_RS09445
• Protein ID: WP_011278760.1
• Coordinates: 1766583..1766810 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACI_RS09415 (Saci_1949)	1762556..1763086	+	531	WP_011278754.1	PaREP1 family protein	-
SACI_RS09420 (Saci_1950)	1763695..1764372	-	678	WP_011278755.1	hypothetical protein	-
SACI_RS12350	1764971..1765096	-	126	WP_257719735.1	hypothetical protein	-
SACI_RS09430 (Saci_1952)	1765335..1765583	+	249	WP_011278757.1	hypothetical protein	-
SACI_RS09435 (Saci_1953)	1765576..1765995	+	420	WP_011278758.1	PIN domain-containing protein	-
SACI_RS09440 (Saci_1954)	1766204..1766599	-	396	WP_011278759.1	PIN domain-containing protein	Toxin
SACI_RS09445 (Saci_1955)	1766583..1766810	-	228	WP_011278760.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SACI_RS09450 (Saci_1956)	1766910..1767353	-	444	WP_011278761.1	MarR family transcriptional regulator	-
SACI_RS09455 (Saci_1957)	1767340..1769043	-	1704	WP_011278762.1	MFS transporter	-
SACI_RS09460 (Saci_1958)	1769620..1770117	+	498	WP_011278763.1	hypothetical protein	-
SACI_RS09465 (Saci_1959)	1770138..1770809	+	672	WP_015385737.1	hypothetical protein	-
SACI_RS11995 (Saci_1960)	1770815..1771582	+	768	WP_011278765.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (3-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15289.79 Da
• Isoelectric Point: 5.7030

>T21212 WP_011278759.1 NC_007181:c1766599-1766204 [Sulfolobus acidocaldarius DSM 639]
MKILVDTNVLIYDLVENSKFHKEAEDILDRAETWVIPSVVVHELVWFLRANNLERMDYVSAYVESPKAEIVCEDFDVLKN
AMEIILREKISLSRYNDPVILSHAILRKLPLSTFDRRLSNLAKRYGIDIVK

Nucleotide

• Length: 396 bp

>T21212 NC_007181:c1766599-1766204 [Sulfolobus acidocaldarius DSM 639]
ATGAAAATTTTAGTTGATACTAACGTACTAATCTATGACCTCGTGGAGAACTCAAAGTTTCACAAGGAAGCTGAGGATAT
ATTGGACAGAGCAGAAACTTGGGTCATACCTTCTGTAGTCGTTCATGAGCTAGTATGGTTCTTGAGGGCGAATAACCTGG
AGAGAATGGACTACGTATCAGCATATGTGGAAAGCCCCAAGGCTGAAATAGTGTGCGAGGACTTTGATGTTTTAAAGAAC
GCAATGGAAATAATACTGAGGGAAAAGATATCTCTTTCTAGGTATAATGACCCAGTAATACTCTCACACGCGATTCTCAG
GAAGTTACCACTTTCGACGTTTGATAGGAGACTCTCCAACTTGGCAAAGAGGTACGGAATAGACATAGTTAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8808.27 Da
• Isoelectric Point: 8.4634

>AT21212 WP_011278760.1 NC_007181:c1766810-1766583 [Sulfolobus acidocaldarius DSM 639]
VEKVKVTRNYQVTIPSSVRNVLGIKEGDILEVYVENDKIVMKKVESKRPRIKIEKLMTIEYIEESIERGMHENFS

Nucleotide

• Length: 228 bp

>AT21212 NC_007181:c1766810-1766583 [Sulfolobus acidocaldarius DSM 639]
GTGGAGAAGGTAAAAGTTACTAGGAACTACCAGGTCACGATACCTTCATCTGTGAGGAACGTCCTAGGAATTAAGGAGGG
TGATATTTTGGAGGTGTACGTAGAGAATGATAAAATAGTGATGAAGAAGGTAGAGTCTAAGAGACCTAGGATAAAGATTG
AAAAACTTATGACCATAGAATATATAGAGGAAAGTATAGAGAGAGGCATGCATGAAAATTTTAGTTGA

Structures

Toxin

• AlphaFold DB: M1ITB5

Antitoxin

• AlphaFold DB: M1I7B9