Detailed information of TA system TA212111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2236470..2236999
• Replicon: chromosome
• Accession: NZ_CP080556
• Organism: Staphylococcus aureus strain HL17078

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMO10_RS11300
• Protein ID: WP_000621175.1
• Coordinates: 2236470..2236832 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMO10_RS11305
• Protein ID: WP_000948331.1
• Coordinates: 2236829..2236999 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO10_RS11280 (2233448)	2233448..2234218	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
JMO10_RS11285 (2234193)	2234193..2234672	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JMO10_RS11290 (2234674)	2234674..2235000	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMO10_RS11295 (2235119)	2235119..2236120	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JMO10_RS11300 (2236470)	2236470..2236832	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMO10_RS11305 (2236829)	2236829..2236999	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMO10_RS11310 (2237084)	2237084..2238232	-	1149	WP_001281145.1	alanine racemase	-
JMO10_RS11315 (2238298)	2238298..2238657	-	360	WP_000581200.1	holo-ACP synthase	-
JMO10_RS11320 (2238661)	2238661..2239152	-	492	WP_001205910.1	PH domain-containing protein	-
JMO10_RS11325 (2239139)	2239139..2240722	-	1584	WP_001294626.1	PH domain-containing protein	-
JMO10_RS11330 (2240715)	2240715..2241194	-	480	WP_001287088.1	hypothetical protein	-
JMO10_RS11335 (2241402)	2241402..2241962	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212111 WP_000621175.1 NZ_CP080556:c2236832-2236470 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212111 NZ_CP106992:c5371557-5371207 [Paenibacillus thiaminolyticus]
TTGATTGTAAAGCGCGGCGACGTTTTTTTTGCGGATTTATCGCCCGTCGTTGGTTCAGAGCAGGGAGGGGTGCGTCCCGT
TCTCGTCATTCAGAATGATATCGGGAACCGGTTCAGCCCAACCGTCATCGTGGCGGCAATTACGGCGCAGATTCAGAAGG
CCAAATTGCCGACCCATGTTGAAATTGATGCGAAGACGCATGGCTTCGACCGCGACTCCGTCATTTTGCTGGAACAGATC
CGCACCATCGACAAACAACGGCTCACTGACAAAATCACGCACTTAGATGAAGAGACGATGCGCAAGGTCGATGACGGGCT
GCTTATCAGCCTCGGATTGATTGATTTTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212111 WP_000948331.1 NZ_CP080556:c2236999-2236829 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212111 NZ_CP106992:c5371842-5371561 [Paenibacillus thiaminolyticus]
GTGGCTAATATTCAGAACACCAAACGAATTATGATCAGCTTGCCCGACCAACTATTGCAAGAAGTGGACGGTATCGTTGC
TATGGAAAAATCGAATCGAAGCGAGTTCATTCGCCAAGCGATGAAGTTGTATTTGCAAGAGCGCAAGAAGCGTCACATCC
GTGAATCGATGCAGCGCGGCTATATGGAAATGGCCAAAATTAACTTGACGATGGCATCCGAGGCTTTTTACGCAGAAGTC
GAAGCGGATGGCACGCTCGACCGCCTGGTTAGCGGGGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1