Detailed information of TA system TA212094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2232900..2233429
• Replicon: chromosome
• Accession: NZ_CP080553
• Organism: Staphylococcus aureus strain HL18380

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMO11_RS11285
• Protein ID: WP_000621175.1
• Coordinates: 2232900..2233262 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMO11_RS11290
• Protein ID: WP_000948331.1
• Coordinates: 2233259..2233429 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO11_RS11265 (2229879)	2229879..2230649	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
JMO11_RS11270 (2230624)	2230624..2231103	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JMO11_RS11275 (2231105)	2231105..2231431	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMO11_RS11280 (2231550)	2231550..2232551	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JMO11_RS11285 (2232900)	2232900..2233262	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMO11_RS11290 (2233259)	2233259..2233429	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMO11_RS11295 (2233514)	2233514..2234662	-	1149	WP_001281145.1	alanine racemase	-
JMO11_RS11300 (2234728)	2234728..2235087	-	360	WP_000581200.1	holo-ACP synthase	-
JMO11_RS11305 (2235091)	2235091..2235582	-	492	WP_001205910.1	PH domain-containing protein	-
JMO11_RS11310 (2235569)	2235569..2237152	-	1584	WP_001294626.1	PH domain-containing protein	-
JMO11_RS11315 (2237145)	2237145..2237624	-	480	WP_001287088.1	hypothetical protein	-
JMO11_RS11320 (2237832)	2237832..2238392	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212094 WP_000621175.1 NZ_CP080553:c2233262-2232900 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212094 NZ_CP106964:1833349-1833681 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTGACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTTACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGTAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGACGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212094 WP_000948331.1 NZ_CP080553:c2233429-2233259 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212094 NZ_CP106964:1833091-1833348 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCTGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1