Detailed information of TA system TA212079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2223193..2223722
• Replicon: chromosome
• Accession: NZ_CP080552
• Organism: Staphylococcus aureus strain HL18807

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMO12_RS11150
• Protein ID: WP_000621175.1
• Coordinates: 2223193..2223555 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMO12_RS11155
• Protein ID: WP_000948331.1
• Coordinates: 2223552..2223722 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO12_RS11130 (JMO12_002228)	2220172..2220942	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
JMO12_RS11135 (JMO12_002229)	2220917..2221396	-	480	WP_044421339.1	anti-sigma B factor RsbW	-
JMO12_RS11140 (JMO12_002230)	2221398..2221724	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMO12_RS11145 (JMO12_002231)	2221843..2222844	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JMO12_RS11150 (JMO12_002232)	2223193..2223555	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMO12_RS11155 (JMO12_002233)	2223552..2223722	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMO12_RS11160 (JMO12_002234)	2223807..2224955	-	1149	WP_001281154.1	alanine racemase	-
JMO12_RS11165 (JMO12_002235)	2225021..2225380	-	360	WP_000581197.1	holo-ACP synthase	-
JMO12_RS11170 (JMO12_002236)	2225384..2225875	-	492	WP_001205912.1	PH domain-containing protein	-
JMO12_RS11175 (JMO12_002237)	2225862..2227445	-	1584	WP_001294626.1	PH domain-containing protein	-
JMO12_RS11180 (JMO12_002238)	2227438..2227917	-	480	WP_001287087.1	hypothetical protein	-
JMO12_RS11185 (JMO12_002239)	2228125..2228685	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212079 WP_000621175.1 NZ_CP080552:c2223555-2223193 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212079 NZ_CP106929:2388105-2388401 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212079 WP_000948331.1 NZ_CP080552:c2223722-2223552 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212079 NZ_CP106929:2388403-2388798 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1