Detailed information of TA system TA212064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2122161..2122690
• Replicon: chromosome
• Accession: NZ_CP080551
• Organism: Staphylococcus aureus strain HL18840

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMO13_RS10520
• Protein ID: WP_000621175.1
• Coordinates: 2122161..2122523 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMO13_RS10525
• Protein ID: WP_000948331.1
• Coordinates: 2122520..2122690 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO13_RS10500 (2119140)	2119140..2119910	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
JMO13_RS10505 (2119885)	2119885..2120364	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JMO13_RS10510 (2120366)	2120366..2120692	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMO13_RS10515 (2120811)	2120811..2121812	-	1002	WP_138390383.1	PP2C family protein-serine/threonine phosphatase	-
JMO13_RS10520 (2122161)	2122161..2122523	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMO13_RS10525 (2122520)	2122520..2122690	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMO13_RS10530 (2122775)	2122775..2123923	-	1149	WP_001281142.1	alanine racemase	-
JMO13_RS10535 (2123989)	2123989..2124348	-	360	WP_000581197.1	holo-ACP synthase	-
JMO13_RS10540 (2124352)	2124352..2124843	-	492	WP_001205910.1	PH domain-containing protein	-
JMO13_RS10545 (2124830)	2124830..2126413	-	1584	WP_001294626.1	PH domain-containing protein	-
JMO13_RS10550 (2126406)	2126406..2126885	-	480	WP_001287090.1	hypothetical protein	-
JMO13_RS10555 (2127094)	2127094..2127654	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212064 WP_000621175.1 NZ_CP080551:c2122523-2122161 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212064 NZ_CP106929:c107509-107222 [Escherichia coli]
ATGTTACCGGTTTTATGGCTCGAAAGCGCAGATGCCGACCTAGATAATATAACCAGCTATATTGCACGTTTTGACATAGA
TGCGGCAGAACGTTTGTGGAAACGCTTAAGGACTTGCGTCCTGCCATTATCTGAACATCCGTATTTATACCCGCCAAGCG
ACAGAGTGCCTGGTTTGCGTGAAACTGTAGCTCACCCCAATTACATCATTCTGTACCGCGTGACAATTTCAGGCATTGAA
ATTGTTAACGTAGTTCACGCCCGCCGACAGTTCCCTGTAACACCTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212064 WP_000948331.1 NZ_CP080551:c2122690-2122520 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212064 NZ_CP106929:c107701-107510 [Escherichia coli]
ATGAACACGGCCCTTTCACCAATGGTTTCTGAATTTGAAACCGTCGAGCAAGAAAATAGTTATAACGAATGGCTGCGTGC
AAAAGTGGCCGCAAGCCTTGCTGATCCACGTCCAGCCATTCCCCATGACGAAGTTGAACGCAGAATGGCTGAACGCTTTG
CTAACATGCGCAAGGAACGGAGCAAACAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1