Detailed information of TA system TA212060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1915514..1916290
• Replicon: chromosome
• Accession: NZ_CP080551
• Organism: Staphylococcus aureus strain HL18840

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: JMO13_RS09245
• Protein ID: WP_000031108.1
• Coordinates: 1915514..1915666 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: JMO13_RS09250
• Protein ID: WP_001251229.1
• Coordinates: 1915691..1916290 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO13_RS09230 (1911430)	1911430..1912251	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
JMO13_RS09235 (1912704)	1912704..1914089	-	1386	WP_000116231.1	class II fumarate hydratase	-
JMO13_RS09240 (1914285)	1914285..1914680	-	396	WP_000901023.1	hypothetical protein	-
JMO13_RS09245 (1915514)	1915514..1915666	-	153	WP_000031108.1	SAS053 family protein	Toxin
JMO13_RS09250 (1915691)	1915691..1916290	-	600	WP_001251229.1	glucosamine-6-phosphate isomerase	Antitoxin
JMO13_RS09255 (1916448)	1916448..1916918	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
JMO13_RS09260 (1916923)	1916923..1918050	-	1128	WP_029160281.1	tRNA epoxyqueuosine(34) reductase QueG	-
JMO13_RS09265 (1918201)	1918201..1918923	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
JMO13_RS09270 (1918916)	1918916..1920373	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T212060 WP_000031108.1 NZ_CP080551:c1915666-1915514 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T212060 NZ_CP106924:3813826-3814104 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22329.45 Da
• Isoelectric Point: 5.1445

>AT212060 WP_001251229.1 NZ_CP080551:c1916290-1915691 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEGLGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT212060 NZ_CP106924:3813563-3813823 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6