Detailed information of TA system TA212050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2223284..2223813
• Replicon: chromosome
• Accession: NZ_CP080550
• Organism: Staphylococcus aureus strain HL18883

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JMO14_RS11140
• Protein ID: WP_000621175.1
• Coordinates: 2223284..2223646 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JMO14_RS11145
• Protein ID: WP_000948331.1
• Coordinates: 2223643..2223813 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMO14_RS11120 (JMO14_002226)	2220263..2221033	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
JMO14_RS11125 (JMO14_002227)	2221008..2221487	-	480	WP_044421339.1	anti-sigma B factor RsbW	-
JMO14_RS11130 (JMO14_002228)	2221489..2221815	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JMO14_RS11135 (JMO14_002229)	2221934..2222935	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JMO14_RS11140 (JMO14_002230)	2223284..2223646	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JMO14_RS11145 (JMO14_002231)	2223643..2223813	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JMO14_RS11150 (JMO14_002232)	2223898..2225046	-	1149	WP_001281154.1	alanine racemase	-
JMO14_RS11155 (JMO14_002233)	2225112..2225471	-	360	WP_000581197.1	holo-ACP synthase	-
JMO14_RS11160 (JMO14_002234)	2225475..2225966	-	492	WP_001205912.1	PH domain-containing protein	-
JMO14_RS11165 (JMO14_002235)	2225953..2227536	-	1584	WP_001294626.1	PH domain-containing protein	-
JMO14_RS11170 (JMO14_002236)	2227529..2228008	-	480	WP_001287087.1	hypothetical protein	-
JMO14_RS11175 (JMO14_002237)	2228216..2228776	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212050 WP_000621175.1 NZ_CP080550:c2223646-2223284 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212050 NZ_CP106924:1808858-1809112 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212050 WP_000948331.1 NZ_CP080550:c2223813-2223643 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212050 NZ_CP106924:1808610-1808861 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1