Detailed information of TA system TA212019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 927934..928436
• Replicon: chromosome
• Accession: NZ_CP080536
• Organism: Acidithiobacillus ferriphilus strain GT2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: K1Y48_RS04590
• Protein ID: WP_215849503.1
• Coordinates: 927934..928188 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A060ULQ1
• Locus tag: K1Y48_RS04595
• Protein ID: WP_035191769.1
• Coordinates: 928185..928436 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1Y48_RS04560 (K1Y48_04580)	923404..924480	+	1077	WP_215849673.1	DUF4263 domain-containing protein	-
K1Y48_RS04565 (K1Y48_04585)	924552..925166	+	615	WP_215849507.1	hypothetical protein	-
K1Y48_RS04570 (K1Y48_04590)	925276..925653	+	378	WP_229066264.1	DUF2513 domain-containing protein	-
K1Y48_RS04575 (K1Y48_04595)	925728..926444	+	717	WP_215849505.1	hypothetical protein	-
K1Y48_RS04580 (K1Y48_04600)	926648..926929	+	282	WP_229066265.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K1Y48_RS04585 (K1Y48_04605)	926940..927236	+	297	WP_215849504.1	HigA family addiction module antitoxin	-
K1Y48_RS04590 (K1Y48_04610)	927934..928188	-	255	WP_215849503.1	Txe/YoeB family addiction module toxin	Toxin
K1Y48_RS04595 (K1Y48_04615)	928185..928436	-	252	WP_035191769.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
K1Y48_RS04600 (K1Y48_04620)	928803..930314	+	1512	WP_215849502.1	PQQ-binding-like beta-propeller repeat protein	-
K1Y48_RS04605 (K1Y48_04625)	930431..931264	+	834	WP_229066266.1	ferritin-like domain-containing protein	-
K1Y48_RS04610 (K1Y48_04630)	931261..932274	+	1014	WP_215849500.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-
K1Y48_RS04615 (K1Y48_04635)	932303..932899	-	597	WP_215849499.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
K1Y48_RS04620 (K1Y48_04640)	932972..933331	+	360	WP_215849498.1	dihydroneopterin aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10194.68 Da
• Isoelectric Point: 9.4809

>T212019 WP_215849503.1 NZ_CP080536:c928188-927934 [Acidithiobacillus ferriphilus]
VRLIFSDDAWEDYLYWQKQDKRVVERINKLIRETQREPFAGVGKPEALKHALSGFWSRRITDEHRMVYRIEGDALMIAQL
RFHY

Nucleotide

• Length: 255 bp

>T212019 NZ_CP106912:355311-355658 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9118.51 Da
• Isoelectric Point: 6.9767

>AT212019 WP_035191769.1 NZ_CP080536:c928436-928185 [Acidithiobacillus ferriphilus]
MDAITYTTVRANLASAMDRVCDDHEPLIITRNGEQSVVMLSLEDYKALEETAYLLRTPANAKRLLAAVAQLNAGKGVERK
LAK

Nucleotide

• Length: 252 bp

>AT212019 NZ_CP106912:355658-355957 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A060ULQ1