Detailed information of TA system TA212006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii(toxin)
• Location: 78091..79558
• Replicon: plasmid pMspAR02c
• Accession: NZ_CP080530
• Organism: Mesorhizobium sp. AR02

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: DBIPINDM_RS00320
• Protein ID: WP_258580963.1
• Coordinates: 78091..78960 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: DBIPINDM_RS00325
• Protein ID: WP_258580964.1
• Coordinates: 78953..79558 (-)
• Length: 202 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBIPINDM_RS00320 (DBIPINDM_007242)	78091..78960	-	870	WP_258580963.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
DBIPINDM_RS00325 (DBIPINDM_007243)	78953..79558	-	606	WP_258580964.1	transcriptional regulator	Antitoxin
DBIPINDM_RS00330 (DBIPINDM_007244)	79711..82755	-	3045	WP_258580965.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	groEL	1..988422	988422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-265)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32201.68 Da
• Isoelectric Point: 6.1504

>T212006 WP_258580963.1 NZ_CP080530:c78960-78091 [Mesorhizobium sp. AR02]
MAKEIKNIGASVRARLLQLAKASGQSFDLVLTRFALERLLFRLSQSRHADRFVLKGAMLMMSWFDDPHRGTRDLDLLGFG
DPSAEPMLATFREILAQDAGDGVEFDADALRVDRIREELEYGGLRRRTTASISGARISLTIDIGFGDALEPGTEDLDYPS
MLDFPMPRLRGYARETVIAEKFQAMVALGRANSRMKDFYDIWILSRSFTFDDDRLPRAISATFARRGTAIPQDLPDALTP
AFAEDAQKQRQWRAFVGDVAHDPGDLGNVTRDIAAFLMRHAVSAASLGK

Nucleotide

• Length: 870 bp

>T212006 NZ_CP106906:4201513-4201731 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 202 a.a.
• Molecular weight: 22027.48 Da
• Isoelectric Point: 10.1316

>AT212006 WP_258580964.1 NZ_CP080530:c79558-78953 [Mesorhizobium sp. AR02]
MSQRQIAHTVLTARGISRLAELREAGVTAATMSRMERDGEVLRLARGLYQLSDAPLDVHHSLAEAAKRVPKGVVCLVSAL
AFHGLTDQLPRQVWLAVGQKDWPPKPDGTPIRIVRFTERLLNDSVETHVIEGVPIKVFGVIKTVADCFRYRNKIGLSVAI
EGLQEALRQRKATPGEIARQAEHGGVSTVIRPYLEALTANG

Nucleotide

• Length: 606 bp

>AT212006 NZ_CP106906:4201112-4201486 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG