Detailed information of TA system TA212004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 407935..408596
• Replicon: plasmid pMspAR07d
• Accession: NZ_CP080528
• Organism: Mesorhizobium sp. AR07

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BPNPMPFG_RS38110
• Protein ID: WP_183455099.1
• Coordinates: 408159..408596 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BPNPMPFG_RS38105
• Protein ID: WP_183455098.1
• Coordinates: 407935..408162 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPNPMPFG_RS38065 (BPNPMPFG_006861)	403224..404150	+	927	WP_258598943.1	ArdC family protein	-
BPNPMPFG_RS38070 (BPNPMPFG_006862)	404368..404676	+	309	WP_183455093.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
BPNPMPFG_RS38075 (BPNPMPFG_006863)	404673..404966	+	294	WP_183455094.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BPNPMPFG_RS38080 (BPNPMPFG_006864)	405608..405919	+	312	WP_183455095.1	DUF736 domain-containing protein	-
BPNPMPFG_RS38085 (BPNPMPFG_006865)	406035..406280	+	246	WP_183455096.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
BPNPMPFG_RS38090 (BPNPMPFG_006866)	406277..406663	+	387	WP_183465714.1	type II toxin-antitoxin system VapC family toxin	-
BPNPMPFG_RS38095 (BPNPMPFG_006867)	407365..407676	+	312	WP_183455095.1	DUF736 domain-containing protein	-
BPNPMPFG_RS38100 (BPNPMPFG_006868)	407738..407914	+	177	WP_183465713.1	hypothetical protein	-
BPNPMPFG_RS38105 (BPNPMPFG_006869)	407935..408162	+	228	WP_183455098.1	CopG family transcriptional regulator	Antitoxin
BPNPMPFG_RS38110 (BPNPMPFG_006870)	408159..408596	+	438	WP_183455099.1	PIN domain-containing protein	Toxin
BPNPMPFG_RS38115 (BPNPMPFG_006871)	408634..408912	-	279	WP_183455100.1	HU family DNA-binding protein	-
BPNPMPFG_RS38120 (BPNPMPFG_006872)	409032..409277	-	246	WP_183465712.1	WGR domain-containing protein	-
BPNPMPFG_RS38125 (BPNPMPFG_006873)	409652..409835	-	184	Protein_398	type IV secretory system conjugative DNA transfer family protein	-
BPNPMPFG_RS38130 (BPNPMPFG_006874)	410526..411488	+	963	WP_258598944.1	TniB family NTP-binding protein	-
BPNPMPFG_RS38135 (BPNPMPFG_006875)	411485..412939	+	1455	WP_258598945.1	TniQ family protein	-
BPNPMPFG_RS38140 (BPNPMPFG_006876)	413004..413342	-	339	WP_258598946.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	kdsA / gmd	1..1021442	1021442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15253.37 Da
• Isoelectric Point: 6.8420

>T212004 WP_183455099.1 NZ_CP080528:408159-408596 [Mesorhizobium sp. AR07]
VTFLLDVNVLIALIDPGHVAHDDAHGWFEVTGYAAWATCPITENGVLRIVGNQKYPNSPGSPAVVAQIVTKLRALHGHSF
WPDDVSLVGSGDIDAAKILTSAQVTDTYLLALAKAHGGQLASFDRKLSAAALKSGKSSLHLITTS

Nucleotide

• Length: 438 bp

>T212004 NZ_CP106906:2012277-2012379 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8186.36 Da
• Isoelectric Point: 6.2820

>AT212004 WP_183455098.1 NZ_CP080528:407935-408162 [Mesorhizobium sp. AR07]
MRTTLAIDDDVLVAAKAMARQQDRSVGEIITDLARRSLRRPQAGGERNGIPLLSPRPDAPPVTLETVNALRDELP

Nucleotide

• Length: 228 bp

>AT212004 NZ_CP106906:c2012386-2012241 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT