Detailed information of TA system TA212001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 48045..48646
• Replicon: plasmid pMspAR07b
• Accession: NZ_CP080527
• Organism: Mesorhizobium sp. AR07

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A6M7Y451
• Locus tag: BPNPMPFG_RS34665
• Protein ID: WP_027054595.1
• Coordinates: 48323..48646 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: BPNPMPFG_RS34660
• Protein ID: WP_006205593.1
• Coordinates: 48045..48326 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPNPMPFG_RS34630 (BPNPMPFG_007818)	43381..43668	-	288	WP_258598023.1	WGR domain-containing protein	-
BPNPMPFG_RS34635 (BPNPMPFG_007819)	43879..44220	-	342	WP_258598024.1	hypothetical protein	-
BPNPMPFG_RS34640 (BPNPMPFG_007820)	44217..44909	-	693	WP_258598025.1	ParA family protein	-
BPNPMPFG_RS34645 (BPNPMPFG_007821)	44802..45116	+	315	WP_258598231.1	hypothetical protein	-
BPNPMPFG_RS34650 (BPNPMPFG_007822)	45235..45645	+	411	WP_258598026.1	hypothetical protein	-
BPNPMPFG_RS34655 (BPNPMPFG_007823)	45642..47912	+	2271	WP_258598027.1	relaxase/mobilization nuclease domain-containing protein	-
BPNPMPFG_RS34660 (BPNPMPFG_007824)	48045..48326	-	282	WP_006205593.1	XRE family transcriptional regulator	Antitoxin
BPNPMPFG_RS34665 (BPNPMPFG_007825)	48323..48646	-	324	WP_027054595.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BPNPMPFG_RS34670 (BPNPMPFG_007826)	48867..51194	-	2328	WP_258598029.1	type IV secretory system conjugative DNA transfer family protein	-
BPNPMPFG_RS34675 (BPNPMPFG_007827)	51172..52218	-	1047	WP_095204880.1	P-type DNA transfer ATPase VirB11	-
BPNPMPFG_RS34680 (BPNPMPFG_007828)	52184..53413	-	1230	WP_258598030.1	type IV secretion system protein VirB10	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..348101	348101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-101)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-90)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12289.12 Da
• Isoelectric Point: 10.1375

>T212001 WP_027054595.1 NZ_CP080527:c48646-48323 [Mesorhizobium sp. AR07]
MKVIEWLGSSRADVRAFPDDARIEAGWQLELVQRGDDPDDWKPMQIVGAGVREIRVREASGAFRVIYLATLEDRVLVLHA
FQKKTQATSKKDLDLAAQRLKRWKAER

Nucleotide

• Length: 324 bp

>T212001 NZ_CP106906:355278-355625 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10483.08 Da
• Isoelectric Point: 7.0148

>AT212001 WP_006205593.1 NZ_CP080527:c48326-48045 [Mesorhizobium sp. AR07]
MNVEIYENVWDALADTEQEAANLKARSALLYEIRKAVQRWGIPQEEAAKRLGLTRPRINDLLRGKLAKFSLDALVNIAAS
AHLDIEIRVKEVA

Nucleotide

• Length: 282 bp

>AT212001 NZ_CP106906:355625-355924 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A6M7Y451