Detailed information of TA system TA211985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3314605..3315332
• Replicon: chromosome
• Accession: NZ_CP080521
• Organism: Pseudonocardia sp. DSM 110487

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K1T35_RS15425
• Protein ID: WP_255621926.1
• Coordinates: 3314841..3315332 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K1T35_RS15420
• Protein ID: WP_220260843.1
• Coordinates: 3314605..3314844 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1T35_RS15395 (K1T35_15395)	3310101..3310880	+	780	WP_220260839.1	ABC transporter permease	-
K1T35_RS15400 (K1T35_15400)	3310877..3311890	+	1014	WP_220260840.1	amidohydrolase family protein	-
K1T35_RS15405 (K1T35_15405)	3312174..3312395	+	222	WP_220260841.1	MbtH family NRPS accessory protein	-
K1T35_RS15410 (K1T35_15410)	3312471..3313310	+	840	WP_255621924.1	alpha/beta fold hydrolase	-
K1T35_RS15415 (K1T35_15415)	3313300..3314559	+	1260	WP_220260842.1	SidA/IucD/PvdA family monooxygenase	-
K1T35_RS15420 (K1T35_15420)	3314605..3314844	+	240	WP_220260843.1	ribbon-helix-helix protein, CopG family	Antitoxin
K1T35_RS15425 (K1T35_15425)	3314841..3315332	+	492	WP_255621926.1	type II toxin-antitoxin system VapC family toxin	Toxin
K1T35_RS15430 (K1T35_15430)	3315336..3316736	-	1401	WP_220260845.1	MFS transporter	-
K1T35_RS15435 (K1T35_15435)	3316826..3317305	+	480	WP_220260846.1	MarR family transcriptional regulator	-
K1T35_RS15440 (K1T35_15440)	3317371..3317610	-	240	WP_220260847.1	hypothetical protein	-
K1T35_RS48845	3318028..3318324	+	297	WP_255621927.1	hypothetical protein	-
K1T35_RS48850	3318390..3319349	+	960	WP_255621928.1	sigma-70 family RNA polymerase sigma factor	-
K1T35_RS15450 (K1T35_15450)	3319353..3319871	-	519	WP_220260849.1	dihydrofolate reductase family protein	-
K1T35_RS15455 (K1T35_15455)	3319864..3320280	-	417	WP_220260850.1	PPOX class F420-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-133)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-37)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17669.98 Da
• Isoelectric Point: 7.5155

>T211985 WP_255621926.1 NZ_CP080521:3314841-3315332 [Pseudonocardia sp. DSM 110487]
VISCDVNVLIYAHNADDPRHGEYARWLRAAVNGDRPFGLSSLVASAFVRIVTHPKVLTKPLDSKQALAAIEPLRSAPAVV
AMEPGPRHWSLFATLCQQVAARGNTVSDAYLAALAIEHDAEWYSADRGFARFPGLRHHHPLDSPADEPPGSSQPTVPPAR
RPS

Nucleotide

• Length: 492 bp

>T211985 NZ_CP106892:6417-6833 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8883.92 Da
• Isoelectric Point: 4.8129

>AT211985 WP_220260843.1 NZ_CP080521:3314605-3314844 [Pseudonocardia sp. DSM 110487]
VRTTITIDEHLLAEVRQVAAARRQTVSQVIEESVRASLLRREQESREPFELRTFHAGGYQAGVDLDDNAALLELMDGHP

Nucleotide

• Length: 240 bp

>AT211985 NZ_CP106892:6190-6420 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA