Detailed information of TA system TA211981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4486134..4487392
• Replicon: chromosome
• Accession: NZ_CP080518
• Organism: Pseudomonas aeruginosa strain YY322

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KMW38_RS20720
• Protein ID: WP_003163133.1
• Coordinates: 4486134..4486889 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KMW38_RS20725
• Protein ID: WP_003086329.1
• Coordinates: 4487024..4487392 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMW38_RS20710 (KMW38_20710)	4481306..4483849	+	2544	WP_003163132.1	acyl-homoserine-lactone acylase QuiP	-
KMW38_RS20715 (KMW38_20715)	4484004..4485365	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
KMW38_RS20720 (KMW38_20720)	4486134..4486889	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
KMW38_RS20725 (KMW38_20725)	4487024..4487392	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KMW38_RS20730 (KMW38_20730)	4487465..4488751	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
KMW38_RS20735 (KMW38_20735)	4488807..4490396	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
KMW38_RS20740 (KMW38_20740)	4490475..4490951	+	477	WP_003163135.1	hypothetical protein	-
KMW38_RS20745 (KMW38_20745)	4490954..4491229	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T211981 WP_003163133.1 NZ_CP080518:c4486889-4486134 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T211981 NZ_CP106890:c4897517-4897233 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT211981 WP_003086329.1 NZ_CP080518:c4487392-4487024 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT211981 NZ_CP106890:c4897749-4897507 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7