Detailed information of TA system TA211974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3085003..3086278
• Replicon: chromosome
• Accession: NZ_CP080512
• Organism: Pseudomonas protegens strain CM-YJ10_15-44

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K1T36_RS14350
• Protein ID: WP_220224264.1
• Coordinates: 3085679..3086278 (+)
• Length: 200 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K1T36_RS14345
• Protein ID: WP_176707103.1
• Coordinates: 3085003..3085560 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1T36_RS14315 (K1T36_14315)	3080256..3080906	+	651	WP_220224260.1	DUF2239 family protein	-
K1T36_RS14320 (K1T36_14320)	3080994..3081614	+	621	WP_220224261.1	LysE family translocator	-
K1T36_RS14325 (K1T36_14325)	3081660..3082247	-	588	WP_019093798.1	AidA/PixA family protein	-
K1T36_RS14330 (K1T36_14330)	3082658..3083845	+	1188	WP_220224262.1	PrpF domain-containing protein	-
K1T36_RS14335 (K1T36_14335)	3084024..3084470	-	447	WP_246881499.1	hypothetical protein	-
K1T36_RS14340 (K1T36_14340)	3084472..3084813	-	342	WP_220224263.1	low molecular weight protein tyrosine phosphatase family protein	-
K1T36_RS14345 (K1T36_14345)	3085003..3085560	+	558	WP_176707103.1	helix-turn-helix transcriptional regulator	Antitoxin
K1T36_RS14350 (K1T36_14350)	3085679..3086278	+	600	WP_220224264.1	HD domain-containing protein	Toxin
K1T36_RS14355 (K1T36_14355)	3086493..3088673	+	2181	WP_220224265.1	catalase/peroxidase HPI	-
K1T36_RS14360 (K1T36_14360)	3088735..3089403	-	669	WP_258571656.1	hypothetical protein	-
K1T36_RS14365 (K1T36_14365)	3089381..3090001	-	621	WP_047286240.1	hypothetical protein	-
K1T36_RS14370 (K1T36_14370)	3090068..3090496	-	429	WP_220224267.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (8-161)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-46)

Sequences

Toxin

Protein

• Length: 200 a.a.
• Molecular weight: 22440.24 Da
• Isoelectric Point: 5.7927

>T211974 WP_220224264.1 NZ_CP080512:3085679-3086278 [Pseudomonas protegens]
MNSELLSGRLDFLREAEKLKDVLRSAHTSSGRQESTAEHSWRLCLMALLFEDQLGDLDLLRVLKLCIVHDLGEAIHGDIP
AVAQAAHPDKSQQERADLQLLARALDAPAREHLLELWDEYEGAASAEAQTVKALDKLETLLQHTQGQNPAGFDYRFNLGY
GRRYTDASPLFQALRQQIDIDTQRRLDAQAQAAVHHRLR

Nucleotide

• Length: 600 bp

>T211974 NZ_CP106890:351915-352262 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20342.24 Da
• Isoelectric Point: 7.0952

>AT211974 WP_176707103.1 NZ_CP080512:3085003-3085560 [Pseudomonas protegens]
MPAAPFTSPHLGLALRRWRLLHRVKQQHAAELFGVAQSSISRWENGQQAMEPAERARLEQLLAANLSAAADQALARLVND
SPRAVHLVCDLTHRLLACSATRAGQFGVPLSELLGHSLWGFCTEEIVRKEAALDDLGWREVLAPPSLEFFSGHNDSAIVP
IAPSQCRWTRLNLSDGNAVRLVETL

Nucleotide

• Length: 558 bp

>AT211974 NZ_CP106890:352262-352561 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA