Detailed information of TA system TA211973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2668107..2668696
• Replicon: chromosome
• Accession: NZ_CP080512
• Organism: Pseudomonas protegens strain CM-YJ10_15-44

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7V8EXE5
• Locus tag: K1T36_RS12280
• Protein ID: WP_019094202.1
• Coordinates: 2668445..2668696 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K1T36_RS12275
• Protein ID: WP_072451388.1
• Coordinates: 2668107..2668448 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1T36_RS12250 (K1T36_12250)	2663451..2664407	-	957	WP_015635066.1	nucleoside-specific channel-forming protein Tsx	-
K1T36_RS12255 (K1T36_12255)	2664670..2665071	+	402	WP_258571643.1	nuclear transport factor 2 family protein	-
K1T36_RS12260 (K1T36_12260)	2665115..2666149	+	1035	WP_015635068.1	selenide, water dikinase SelD	-
K1T36_RS12265 (K1T36_12265)	2666149..2667252	+	1104	WP_220224128.1	tRNA 2-selenouridine(34) synthase MnmH	-
K1T36_RS12270 (K1T36_12270)	2667305..2667646	-	342	WP_053157594.1	DUF3077 domain-containing protein	-
K1T36_RS12275 (K1T36_12275)	2668107..2668448	-	342	WP_072451388.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K1T36_RS12280 (K1T36_12280)	2668445..2668696	-	252	WP_019094202.1	type II toxin-antitoxin system HicA family toxin	Toxin
K1T36_RS12285 (K1T36_12285)	2668784..2669839	-	1056	WP_053157597.1	permease	-
K1T36_RS12290 (K1T36_12290)	2670254..2671030	-	777	WP_011060579.1	IclR family transcriptional regulator	-
K1T36_RS12295 (K1T36_12295)	2671202..2671972	+	771	WP_220224129.1	ABC transporter substrate-binding protein	-
K1T36_RS12300 (K1T36_12300)	2672033..2672689	+	657	WP_011060581.1	amino acid ABC transporter permease	-
K1T36_RS12305 (K1T36_12305)	2672686..2673432	+	747	WP_011060582.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.70 Da
• Isoelectric Point: 10.4707

>T211973 WP_019094202.1 NZ_CP080512:c2668696-2668445 [Pseudomonas protegens]
VSKNDKLLAKLLNDRGTFSWQELVTLLKRLGYTPLEGSGSRVKFDNGDPGAMINLHKPHPGNELKAYIRRQVIEQLKSGG
LIP

Nucleotide

• Length: 252 bp

>T211973 NZ_CP106890:325944-326312 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12438.82 Da
• Isoelectric Point: 4.8281

>AT211973 WP_072451388.1 NZ_CP080512:c2668448-2668107 [Pseudomonas protegens]
VNNLLQHKGYYGSIEVSTEDNCLFGKLQFIRALVSYEGQTVAELEAAFKEAVDDYLDTCEQSGTAPEIPCKGSFNVRVGH
DLHLAAAVAATRQNVSLNDLTRQALNEYLQHHG

Nucleotide

• Length: 342 bp

>AT211973 NZ_CP106890:325726-325947 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A7V8EXE5