Detailed information of TA system TA211971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 756507..757103
• Replicon: chromosome
• Accession: NZ_CP080512
• Organism: Pseudomonas protegens strain CM-YJ10_15-44

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q4KIY8
• Locus tag: K1T36_RS03335
• Protein ID: WP_011059021.1
• Coordinates: 756801..757103 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q4KIY9
• Locus tag: K1T36_RS03330
• Protein ID: WP_011059020.1
• Coordinates: 756507..756797 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1T36_RS03310 (K1T36_03310)	752777..754909	-	2133	WP_011059016.1	TonB-dependent copper receptor	-
K1T36_RS03315 (K1T36_03315)	754998..755408	-	411	WP_065364904.1	DUF2946 domain-containing protein	-
K1T36_RS03320 (K1T36_03320)	755464..755958	-	495	WP_011059018.1	copper chaperone PCu(A)C	-
K1T36_RS03325 (K1T36_03325)	756008..756406	-	399	WP_011059019.1	DUF2946 domain-containing protein	-
K1T36_RS03330 (K1T36_03330)	756507..756797	-	291	WP_011059020.1	putative addiction module antidote protein	Antitoxin
K1T36_RS03335 (K1T36_03335)	756801..757103	-	303	WP_011059021.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K1T36_RS03340 (K1T36_03340)	757287..758015	-	729	WP_011059022.1	cobalt-precorrin-6A reductase	-
K1T36_RS03345 (K1T36_03345)	758012..759109	-	1098	WP_220223632.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
K1T36_RS03350 (K1T36_03350)	759203..760417	-	1215	WP_065364907.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
K1T36_RS03355 (K1T36_03355)	760556..761869	+	1314	WP_220223633.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-97)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11499.32 Da
• Isoelectric Point: 10.4481

>T211971 WP_011059021.1 NZ_CP080512:c757103-756801 [Pseudomonas protegens]
MFVFEQTPEFSRWLLNLKDPQGKTRVLARIRAAQLGRFGDCEPVGEGVHEIRIHRGPGYRVYFSRHGKMIYLLLIGGDKA
TQKRDIQRARQLACDLKSEG

Nucleotide

• Length: 303 bp

>T211971 NZ_CP106888:6417-6833 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10423.94 Da
• Isoelectric Point: 5.0939

>AT211971 WP_011059020.1 NZ_CP080512:c756797-756507 [Pseudomonas protegens]
MRTPVTRFDAAEYLKTPEDMVAYLDACFEEDTGDGVLIRAALNDIARARGMTQVARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKINVSNA

Nucleotide

• Length: 291 bp

>AT211971 NZ_CP106888:6190-6420 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: Q4KIY8

Antitoxin

• AlphaFold DB: Q4KIY9