Detailed information of TA system TA211962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 5071533..5072538
• Replicon: chromosome
• Accession: NZ_CP080510
• Organism: Mycolicibacterium farcinogenes strain BKK/CU-MFGLA-001

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: K1X22_RS24230
• Protein ID: WP_221882537.1
• Coordinates: 5071960..5072538 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A378T1G9
• Locus tag: K1X22_RS24225
• Protein ID: WP_019346253.1
• Coordinates: 5071533..5071922 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1X22_RS24210 (K1X22_24210)	5068157..5068567	-	411	WP_019346256.1	transglycosylase family protein	-
K1X22_RS24215 (K1X22_24215)	5068966..5070321	-	1356	WP_221850776.1	alpha/beta hydrolase-fold protein	-
K1X22_RS24220 (K1X22_24220)	5070449..5071465	+	1017	WP_221882536.1	TIGR03617 family F420-dependent LLM class oxidoreductase	-
K1X22_RS24225 (K1X22_24225)	5071533..5071922	+	390	WP_019346253.1	hypothetical protein	Antitoxin
K1X22_RS24230 (K1X22_24230)	5071960..5072538	+	579	WP_221882537.1	zeta toxin family protein	Toxin
K1X22_RS24235 (K1X22_24235)	5072559..5074814	-	2256	WP_085141546.1	catalase/peroxidase HPI	-
K1X22_RS24240 (K1X22_24240)	5074853..5075284	-	432	WP_036387326.1	Fur family transcriptional regulator	-
K1X22_RS24245 (K1X22_24245)	5075375..5076010	+	636	WP_221882538.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
K1X22_RS24250 (K1X22_24250)	5076063..5076668	+	606	WP_036387323.1	class I SAM-dependent methyltransferase	-
K1X22_RS24255 (K1X22_24255)	5076622..5077461	-	840	WP_109789989.1	thioesterase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-140)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 20924.06 Da
• Isoelectric Point: 7.0515

>T211962 WP_221882537.1 NZ_CP080510:5071960-5072538 [Mycolicibacterium farcinogenes]
VKRLDLVVGCNGAGKSTFVELTLSPLLPGSPFVNADEIAKRRWPEDPAPHSYEAALIAAEIRAKLIELGESFIAETVFSH
PSKLDLIDDAHIAGYTVVLHCILVPEELAVQRVRHRVSAGGHDVPEAKIRERYQRLWDLVAVAAMRADAATFYDNSTVRG
PRIVAQIAGGFAIGSPTWPKWAPAVLISRWAG

Nucleotide

• Length: 579 bp

>T211962 NZ_CP106886:907677-908087 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13745.15 Da
• Isoelectric Point: 5.2329

>AT211962 WP_019346253.1 NZ_CP080510:5071533-5071922 [Mycolicibacterium farcinogenes]
MTETADRVTRFAADLIDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHSAARRKVESALAGELSLRELTAEEGTVFDAEI
AASIEESLARADYGKTLAGRGITTVALDDNGNIVQYRPDGSTAVLARTQ

Nucleotide

• Length: 390 bp

>AT211962 NZ_CP106886:907430-907696 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A378T1G9