Detailed information of TA system TA211961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 719134..719766
• Replicon: chromosome
• Accession: NZ_CP080510
• Organism: Mycolicibacterium farcinogenes strain BKK/CU-MFGLA-001

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: K1X22_RS03540
• Protein ID: WP_019347713.1
• Coordinates: 719134..719583 (-)
• Length: 150 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A378SW37
• Locus tag: K1X22_RS03545
• Protein ID: WP_019347714.1
• Coordinates: 719587..719766 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1X22_RS03515 (K1X22_03515)	714189..715409	+	1221	WP_221883261.1	cytochrome P450	-
K1X22_RS03520 (K1X22_03520)	715406..716455	+	1050	WP_221883262.1	dihydrodipicolinate reductase	-
K1X22_RS03525 (K1X22_03525)	716448..717506	+	1059	WP_221883263.1	dihydrodipicolinate reductase	-
K1X22_RS03530 (K1X22_03530)	717503..717916	+	414	WP_221883264.1	hypothetical protein	-
K1X22_RS03535 (K1X22_03535)	717909..719126	+	1218	WP_221883265.1	cytochrome P450	-
K1X22_RS03540 (K1X22_03540)	719134..719583	-	450	WP_019347713.1	SRPBCC family protein	Toxin
K1X22_RS03545 (K1X22_03545)	719587..719766	-	180	WP_019347714.1	antitoxin	Antitoxin
K1X22_RS03550 (K1X22_03550)	719825..722317	-	2493	WP_085141494.1	cation-translocating P-type ATPase	-
K1X22_RS03555 (K1X22_03555)	722384..723499	-	1116	WP_221883266.1	MBL fold metallo-hydrolase	-
K1X22_RS03560 (K1X22_03560)	723501..724235	-	735	WP_065064412.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 150 a.a.
• Molecular weight: 16121.66 Da
• Isoelectric Point: 9.7310

>T211961 WP_019347713.1 NZ_CP080510:c719583-719134 [Mycolicibacterium farcinogenes]
MAKLSVSVEVPLPPDKAWEHASDLSRYDEWLSIHRAWRSKLPETLEKGTVIDSIVEVKGMLNRVKWTLVNYKPPQSLTLN
GDGRGGVKVKLIGKITPATVDGADGAKVSFDVHLGGPALFGPIGMVVAAALKGDIQQSLNKFKELYASS

Nucleotide

• Length: 450 bp

>T211961 NZ_CP106886:772483-772968 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6529.21 Da
• Isoelectric Point: 4.7045

>AT211961 WP_019347714.1 NZ_CP080510:c719766-719587 [Mycolicibacterium farcinogenes]
MGFLDKAKDLLSQNADKVEQAIDKAGDIVDEKTQGKYSQHVDKAQEAAKNALNKEEPQQ

Nucleotide

• Length: 180 bp

>AT211961 NZ_CP106886:772193-772486 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A378SW37