Detailed information of TA system TA211960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1224298..1225214
• Replicon: chromosome
• Accession: NZ_CP080509
• Organism: Bacillus sp. 7D3

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KTJ85_RS06300
• Protein ID: WP_065981257.1
• Coordinates: 1224468..1225214 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KTJ85_RS06295
• Protein ID: WP_003154807.1
• Coordinates: 1224298..1224468 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTJ85_RS06265 (KTJ85_06265)	1221164..1221544	+	381	WP_065981254.1	XkdW family protein	-
KTJ85_RS06270 (KTJ85_06270)	1221549..1221746	+	198	WP_013351962.1	XkdX family protein	-
KTJ85_RS06275 (KTJ85_06275)	1221794..1222555	+	762	WP_212101067.1	phage portal protein	-
KTJ85_RS06280 (KTJ85_06280)	1222609..1222872	+	264	WP_013351964.1	hemolysin XhlA family protein	-
KTJ85_RS06285 (KTJ85_06285)	1222886..1223149	+	264	WP_013351965.1	phage holin	-
KTJ85_RS06290 (KTJ85_06290)	1223163..1224041	+	879	WP_212101071.1	N-acetylmuramoyl-L-alanine amidase	-
KTJ85_RS19530	1224076..1224201	-	126	WP_003154809.1	hypothetical protein	-
KTJ85_RS06295 (KTJ85_06295)	1224298..1224468	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KTJ85_RS06300 (KTJ85_06300)	1224468..1225214	-	747	WP_065981257.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KTJ85_RS06305 (KTJ85_06305)	1225322..1226320	-	999	WP_065981258.1	inorganic phosphate transporter	-
KTJ85_RS06310 (KTJ85_06310)	1226333..1226950	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KTJ85_RS06315 (KTJ85_06315)	1227235..1228551	-	1317	WP_065981259.1	amino acid permease	-
KTJ85_RS06320 (KTJ85_06320)	1228875..1229825	+	951	WP_115997110.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29041.53 Da
• Isoelectric Point: 4.6839

>T211960 WP_065981257.1 NZ_CP080509:c1225214-1224468 [Bacillus sp. 7D3]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQTRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T211960 NZ_CP106886:351904-352251 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT211960 WP_003154807.1 NZ_CP080509:c1224468-1224298 [Bacillus sp. 7D3]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT211960 NZ_CP106886:352251-352550 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14