Detailed information of TA system TA211959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 469907..470544
• Replicon: chromosome
• Accession: NZ_CP080509
• Organism: Bacillus sp. 7D3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KTJ85_RS02270
• Protein ID: WP_003156187.1
• Coordinates: 470194..470544 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KTJ85_RS02265
• Protein ID: WP_003156188.1
• Coordinates: 469907..470188 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTJ85_RS02245 (KTJ85_02245)	466268..466867	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
KTJ85_RS02250 (KTJ85_02250)	466960..467325	+	366	WP_115998347.1	holo-ACP synthase	-
KTJ85_RS19445	467490..468497	+	1008	WP_065980767.1	outer membrane lipoprotein carrier protein LolA	-
KTJ85_RS02260 (KTJ85_02260)	468619..469788	+	1170	WP_115998346.1	alanine racemase	-
KTJ85_RS02265 (KTJ85_02265)	469907..470188	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KTJ85_RS02270 (KTJ85_02270)	470194..470544	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KTJ85_RS02275 (KTJ85_02275)	470665..471486	+	822	WP_065980769.1	STAS domain-containing protein	-
KTJ85_RS02280 (KTJ85_02280)	471491..471856	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KTJ85_RS02285 (KTJ85_02285)	471859..472260	+	402	WP_065980770.1	anti-sigma regulatory factor	-
KTJ85_RS02290 (KTJ85_02290)	472272..473279	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
KTJ85_RS02295 (KTJ85_02295)	473343..473672	+	330	WP_014469786.1	anti-sigma factor antagonist	-
KTJ85_RS02300 (KTJ85_02300)	473669..474151	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
KTJ85_RS02305 (KTJ85_02305)	474117..474905	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
KTJ85_RS02310 (KTJ85_02310)	474905..475507	+	603	WP_065980772.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T211959 WP_003156187.1 NZ_CP080509:470194-470544 [Bacillus sp. 7D3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T211959 NZ_CP106886:325933-326301 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT211959 WP_003156188.1 NZ_CP080509:469907-470188 [Bacillus sp. 7D3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT211959 NZ_CP106886:325715-325936 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5